EARLY GAMMA INTERFERON-PRODUCTION BY NATURAL-KILLER-CELLS IS IMPORTANT IN DEFENSE AGAINST MURINE LISTERIOSIS
EARLY GAMMA INTERFERON-PRODUCTION BY NATURAL-KILLER-CELLS IS IMPORTANT IN DEFENSE AGAINST MURINE LISTERIOSIS
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DOI：
10.1128/iai.59.9.2892-2900.1991
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发表时间：
1991-09-01
影响因子：
3.1
通讯作者：
NORTH, RJ
中科院分区：
文献类型：
--
作者：
DUNN, PL;NORTH, RJ
用斑点酶联免疫吸附试验表明，皮下接种一定数量的单核细胞增生性李斯特氏菌后，引流淋巴结中产生干扰素的细胞提前出现。相比之下，接种紫外线灭活的单核细胞增多性李斯特菌未能引起产生干扰素-伽马的细胞的出现。干扰素-γ分泌细胞对活体的反应在感染后24小时达到高峰，然后下降。NK1.1、asialo-GM-1和Thy-1标记阳性，而CD4和CD8 T细胞亚群标记阴性。用抗NK细胞抗体治疗早期消除自然杀伤(NK)细胞会导致严重的感染恶化，用大鼠抗小鼠干扰素-γ单抗治疗早期中和内源性干扰素-γ也是如此。相比之下，CD4+和CD8+T细胞的耗尽未能加剧感染。结果表明，早期由NK细胞而不是T细胞产生的干扰素-γ是李斯特菌耐药的重要事件。
A spot enzyme-linked immunosorbent assay was used to show that subcutaneous inoculation of a sublethal number of Listeria monocytogenes resulted in the early appearance of gamma interferon (IFN-gamma)-producing cells in the draining lymph nodes. In contrast, inoculation of UV-killed L. monocytogenes failed to cause the appearance of IFN-gamma-producing cells. The appearance of IFN-gamma-secreting cells in response to the living organisms peaked at 24 h of infection and then declined. The draining lymph node cells responsible for secreting IFN-gamma belonged to a cell population that was positive for the NK1.1, asialo-GM-1, and Thy-1 markers but negative for the CD4 and CD8 T cell subset markers. Early elimination of natural killer (NK) cells by treatment with anti-NK cell antibodies resulted in severe exacerbation of infection, as did early neutralization of endogenous IFN-gamma by treatment with a rat anti-murine IFN-gamma monoclonal antibody. In contrast, depletion of CD4+ and CD8+ T cells failed to exacerbate infection. The results serve to show that the early production of IFN-gamma by NK cells, rather than by T cells, is an essential event in resistance to listeriosis.