SOX gene expression in human osteoarthritic cartilage
SOX gene expression in human osteoarthritic cartilage
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DOI：
10.1159/000124980
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发表时间：
2008-01-01
期刊：
影响因子：
5
通讯作者：
Aigner, Thomas
中科院分区：
文献类型：
--
作者：
Haag, Jochen;Gebhard, Pia M.;Aigner, Thomas
目的：虽然SOX转录因子在胎儿软骨细胞分化中的发育作用已被充分证实，但对于SOX家族成员在正常和骨关节炎成人软骨中的表达知之甚少。因此，本研究的目的是全面分析SOX基因在正常和骨关节炎成人软骨中的表达。方法：采用基因芯片技术（Affymetrix）和实时荧光定量PCR技术，对成人正常膝关节软骨和骨关节炎膝关节软骨的RNA进行基因表达谱分析。结果：大多数SOX转录因子家族成员在成人正常软骨和骨关节炎软骨中不表达或表达水平极低。相比之下，SOX9在正常软骨中的表达相当高，约占GAPDH水平的20%。骨关节炎患者SOX9转录水平显著降低。SOX6水平降低，尽管从正常组织中的低基础表达开始。结论：目前的数据表明，SOX转录因子家族在成人软骨中的作用很可能仅限于少数成员，其中SOX9最为突出。此外，骨关节炎软骨细胞中SOX9和SOX6转录物水平的降低可能是骨关节炎软骨细胞表型稳定性丧失的原因。版权所有2008 S. Karger AG，巴塞尔
Objective: While the developmental role of the SOX transcription factors in fetal chondrocyte differentiation is well documented, much less is known about the expression of SOX family members in normal and osteoarthritic adult cartilage. Therefore, the aim of the present study was to present a thorough analysis of SOX gene expression in normal and osteoarthritic human adult cartilage. Methods: RNA from normal and osteoarthritic knee cartilage from human adults was analyzed by gene expression profiling using GeneChip technology (Affymetrix) and quantitative real time PCR. Results: Most members of the SOX transcription factor family showed no or very low expression levels in normal and osteoarthritic cartilage from adults. In contrast, SOX9 expression was fairly high in normal cartilage, amounting to approximately 20% of GAPDH levels. SOX9 transcript levels were substantially reduced in osteoarthritis. SOX6 levels were reduced, albeit starting from a low basis expression in normal tissue. Conclusion: The presented data indicate that the role of the SOX transcription factor family in adult human cartilage is most probably restricted to a few members, with SOX9 being the most prominent. Furthermore, the reduction of SOX9 and SOX6 transcript levels in osteoarthritic chondrocytes might be responsible for the loss of phenotypic stability of osteoarthritic chondrocytes. Copyright (C) 2008 S. Karger AG, Basel.