Age- and cell cycle-dependent changes in EPC-1/PEDF promoter activity in human diploid fibroblast-like (HDF) cells
Age- and cell cycle-dependent changes in EPC-1/PEDF promoter activity in human diploid fibroblast-like (HDF) cells
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DOI：
10.1007/s11010-006-2680-0
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发表时间：
2006-12-01
影响因子：
4.3
通讯作者：
Morita, Ikuo
中科院分区：
文献类型：
--
作者：
Kojima, Toshihiko;Nakahama, Ken-ichi;Morita, Ikuo
衰老过程中基因表达的变化非常有趣。早期群体倍增cDNA-1（EPC-1，也称为色素上皮衍生因子，PEDF）是细胞衰老过程中表达显著下降的基因之一。我们研究了EPC-1/PEDF启动子活性是否受到细胞老化的影响，使用人二倍体肺成纤维细胞培养。结果表明，EPC-1/PEDF基因的启动子/增强子区位于转录起始位点上游1760 bp以上，受衰老和细胞周期调控。这些发现表明，EPC-1/PEDF基因的表达，至少部分，在细胞中的转录调控。本文对EPC-1/PEDF基因启动子区的分析表明，特定转录因子的表达具有年龄和细胞周期依赖性。
The changes in gene expression during senescence are very interesting. Early population doubling cDNA-1 (EPC-1, also known as pigment epithelial derived factor, PEDF) is one of the genes whose expression decreases dramatically during cellular aging. We examined whether or not EPC-1/PEDF promoter activity was affected by the cellular ageing using human diploid lung fibroblast cells in culture. Here we showed the promoter/enhancer region of EPC-1/PEDF existed at more than 1760 bp upstream from the transcriptional initiation site of the gene, and was regulated by both aging and cell cycle. These findings suggest that the expression of the EPC-1/PEDF gene is, at least in part, regulated transcriptionally in the cells. The analysis of the promoter region of the EPC-1/PEDF gene in this paper suggests the age- and cell cycle-dependent expression of specific transcriptional factor(s).