Expression of miR-126 and Crk in endometriosis: miR-126 may affect the progression of endometriosis by regulating Crk expression
Expression of miR-126 and Crk in endometriosis: miR-126 may affect the progression of endometriosis by regulating Crk expression
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子宫内膜异位症中miR-126和Crk的表达：miR-126可能通过调节Crk表达影响子宫内膜异位症的进展
DOI：
10.1007/s00404-011-2112-6
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发表时间：
2012-04-01
影响因子：
2.6
通讯作者：
Wang, Dan Bo
中科院分区：
文献类型：
--
作者：
Liu, Shuang;Gao, Shan;Wang, Dan Bo
目的评价miR-126与Crk的关系，探讨miR-126在子宫内膜异位症（EMs）发生、发展中的作用。方法采用实时荧光定量聚合酶链反应（real time PCR）对EMs患者异位子宫内膜（ECs）和在位子宫内膜（EUs）及正常子宫内膜（ENs）中miR-126和Crk mRNA的表达水平进行定量。无 EM 的受试者。通过Western blot检测所有样品中Crk蛋白的表达水平。结果实验组中ECs与EUs (p= 5.45E−5)以及EUs与ENs (p= 0.019)中miR-126的表达水平显着下调。 Crk mRNA 的表达水平无法区分 EC 和 EU (p= 0.995)，但与 EN 相比，EU 中 Crk mRNA 过表达 (p= 0.006)。在实验组中，Crk 蛋白在 EC 与 EU 中过度表达 (p= 0.002)，在 EU 与 EN 中过度表达 (p= 1.13E−6)。 miR-126 的表达水平与 Crk mRNA 不相关（p= 0.496），但与 Crk 蛋白呈负相关（p= 3.134E−5）。 EU和EC中miR-126的表达水平与美国生育协会（AFS）分期（p=0.022，p=0.025）和AFS评分（p=0.002，p=0.007）呈负相关。 miR-126表达随着EMs的进展而降低，但降低幅度无显着差异。结论miR-126可能在EMs的发生、发展过程中起初步作用。 Crk可能受miR-126调控，异常表达之间的协同作用可能在EMs的发病机制中发挥重要作用。
PurposeTo evaluate the relationship between miR-126 and Crk and discuss the role of miR-126 in the development and progression of endometriosis (EMs).MethodsThe expression levels of miR-126 and Crk mRNA were quantified using real time fluorescent quantitative polymerase chain reaction (real time PCR) in ectopic endometrium (ECs) and eutopic endometrium (EUs) in patients with EMs and normal endometrium (ENs) in EMs-free subjects. The expression levels of Crk protein in all samples were evaluated by Western blot.ResultsThe expression level of miR-126 was significantly downregulated in ECs versus EUs (p= 5.45E−5) in the experimental group and in EUs versus ENs (p= 0.019). The expression level of Crk mRNA did not distinguish ECs from EUs (p= 0.995) but was overexpressed in EUs versus ENs (p= 0.006). Crk protein was overexpressed in ECs versus EUs (p= 0.002) in the experimental group and in EUs versus ENs (p= 1.13E−6). The expression level of miR-126 had no correlation with Crk mRNA (p= 0.496) but was negatively correlated with Crk protein (p= 3.134E−5). The expression level of miR-126 in EUs and ECs was negatively correlated with American Fertility Society (AFS) stage (p= 0.022,p= 0.025) and AFS score (p= 0.002,p= 0.007). miR-126 expression decreased with the progression of EMs, but the decrease was not significantly different.ConclusionsmiR-126 may play an initial role in the development and progression of EMs. Crk may be regulated by miR-126, and synergism between abnormal expressions may play an important role in the pathogenesis of EMs.