Impact of postharvest brassinosteroids treatment on PAL activity in tomato fruit in response to chilling stress
Impact of postharvest brassinosteroids treatment on PAL activity in tomato fruit in response to chilling stress
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DOI：
10.1016/j.scienta.2012.07.008
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发表时间：
2012-09-06
影响因子：
4.3
通讯作者：
Moradbeygi, Hanieh
中科院分区：
文献类型：
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作者：
Aghdam, Morteza Soleimani;Asghari, Mohammadreza;Moradbeygi, Hanieh
番茄作为热带水果，由于存在冷害（CI）的风险，不易在低温下储存。为研究降低番茄果实冷害的有效技术，以1 ℃贮藏21 d的番茄为试材，研究了0、3和6 μ M油菜素内酯（BR）处理对番茄果实冷害、电解质渗漏、丙二醛（MDA）、脯氨酸和总酚含量以及苯丙氨酸解氨酶（PAL）活性的影响。油菜素内酯处理显著降低了冷害、电解质渗漏和丙二醛含量，尤其是6 μ M处理。此外，与对照相比，BR处理的果实表现出显着较高的总酚和脯氨酸含量。我们的研究结果表明，在贮藏期间与BR处理的水果表现出显着较高的PAL活性。这些结果表明，BR处理保护番茄CI通过提高总酚和脯氨酸含量，降低MDA含量，从而保持膜的完整性。(C)2012爱思唯尔有限公司版权所有。
Tomatoes, as originally tropical fruits, cannot easily be stored at low temperatures, due to the risk of chilling injury (CI). To develop an effective technique to reduce CI, the effects of treatments with 0, 3 and 6 mu M brassinosteroids (BRs) on chilling injury, electrolyte leakage, malondialdehyde (MDA), proline and total phenol contents, and phenylalanine ammonia-lyase (PAL) activity were investigated in tomato fruits stored at 1 degrees C for 21 days. The chilling injury, electrolyte leakage and malondialdehyde content were significantly reduced by brassinosteroids treatments especially at 6 mu M. Also, fruits treated with BRs exhibited significantly higher total phenol and proline contents as compared with the control fruits. Our results indicate that during storage time fruits treated with BRs exhibited significantly higher PAL activity. These results suggest that BRs treatment protects tomatoes from CI by enhancing total phenol and proline contents and reducing MDA content, and thus maintaining membrane integrity. (C) 2012 Elsevier B.V. All rights reserved.