Inheritance and InDel markers closely linked to petal color gene (cpc-1) in Brassica oleracea
Inheritance and InDel markers closely linked to petal color gene (cpc-1) in Brassica oleracea
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与甘蓝花瓣颜色基因（cpc-1）密切相关的遗传和插入缺失标记
DOI：
10.1007/s11032-015-0354-x
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发表时间：
2015-08-01
影响因子：
3.1
通讯作者：
Zhang, Yang-yong
中科院分区：
文献类型：
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作者：
Han, Feng-qing;Yang, Chong;Zhang, Yang-yong
摘要 甘蓝（Brassica oleracea）包含多个亚种，包括卷心菜、西兰花、花椰菜、羽衣甘蓝和大头菜。甘蓝的花瓣是黄色或白色的。卷心菜通常有黄色花瓣，而羽衣甘蓝大多有白色花瓣。然而，甘蓝花瓣颜色形成的分子机制尚不清楚。在本研究中，F2 和 BC1P1（回交）群体是由甘蓝自交系 YL-1（P1）和羽衣甘蓝自交系 11-192（P2）之间的杂交构建的。对花瓣颜色遗传的分析表明，白色花瓣颜色是由一个显性基因（称为 cpc-1）控制的。插入删除（InDel）标记是根据甘蓝参考基因组序列和亲本的重测序数据设计的。随后，使用批量分离分析来检测InDels的多态性。由10个InDels构建的遗传图谱表明cpc-1位于染色体C03上。 cpc-1 基因两侧为 InDels M4136 和 M4139，遗传距离分别为 0.4 和 0.9 cM。两个标记之间的间隔距离为 503 kb。
AbstractBrassica oleracea contains several subspecies, including cabbage, broccoli, cauliflower, Chinese kale and kohlrabi. The petals of B. oleracea are either yellow or white. Cabbages usually have yellow petals, while Chinese kale mostly has white petals. However, the molecular mechanism of petal color formation in B. oleracea is unknown. In this study, F2 and BC1P1 (backcross) populations were constructed from a cross between the cabbage inbred line YL-1 (P1) and the Chinese kale inbred line 11-192 (P2). Analysis of the inheritance of petal color indicated that white petal color was controlled by a single dominant gene, termed cpc-1. Insertion–deletion (InDel) markers were designed based on B. oleracea reference genome sequence and re-sequencing data of the parents. Subsequently, bulked segregation analysis was used to detect polymorphisms of the InDels. A genetic map constructed with 10 InDels indicated that cpc-1 was located on chromosome C03. The cpc-1 gene is flanked by InDels M4136 and M4139, with genetic distances of 0.4 and 0.9 cM, respectively. The interval distance between the two markers is 503 kb.