MiR-129-5p inhibits liver cancer growth by targeting calcium calmodulin-dependent protein kinase IV (CAMK4)
MiR-129-5p inhibits liver cancer growth by targeting calcium calmodulin-dependent protein kinase IV (CAMK4)
复制标题
MiR-129-5p 通过靶向钙调蛋白依赖性蛋白激酶 IV (CAMK4) 抑制肝癌生长
DOI：
10.1038/s41419-019-1923-4
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发表时间：
2019-10-17
影响因子：
9
通讯作者：
Zhang, Jianfeng
中科院分区：
文献类型：
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作者：
Li, Zhengzhao;Lu, Junyu;Zhang, Jianfeng
本研究旨在探讨miR-129- 5 p影响肝癌细胞生物学功能的机制。分别测定肝癌组织和肝癌细胞中miR-129- 5 p的表达水平。结晶紫染色和流式细胞术检测细胞增殖和凋亡。伤口愈合实验和transwell实验检测细胞迁移和侵袭能力。通过生物信息学分析和荧光素酶报告基因分析验证miR-129- 5 p的靶基因。采用裸鼠成瘤实验检测钙调素依赖性蛋白激酶IV（CAMK 4）的抗肿瘤活性。发现miR-129- 5 p在肝细胞癌组织和细胞中低表达，并且还抑制肝细胞增殖、迁移和侵袭并促进凋亡。CAMK 4是miR-129- 5 p的直接靶点，在肝癌组织和细胞中低表达。CAMK 4还可抑制肝细胞增殖、迁移和侵袭，促进细胞凋亡。CAMK 4可能通过抑制丝裂原活化蛋白激酶（MARK）的活化而发挥抗肿瘤作用。miR-129- 5 p在肝癌组织和细胞中呈低表达，是一种抑癌基因。CAMK 4是miR-129- 5 p的直接靶基因，通过抑制MAPK信号通路的激活而发挥抑瘤作用。
This study was designed to investigate the mechanism by which miR-129-5p affects the biological function of liver cancer cells. The expression levels of miR-129-5p in liver cancer tissues and cells were, respectively, determined. Crystal violet staining and flow cytometry were used to detect cell proliferation and apoptosis. Wound healing assay and transwell assay were performed to test cell migration and invasion. The target gene of miR-129-5p was analyzed and verified by bioinformatics analysis and luciferase reporter assay. Tumorigenicity assays in nude mice were used to test the antitumor ability of calcium calmodulin-dependent protein kinase IV (CAMK4). miR-129-5p was found to be underexpressed in hepatocellular cancer tissues and cells and also to inhibit liver cells proliferation, migration, and invasion and promote apoptosis. CAMK4 was a direct target for miR-129-5p and was lowly expressed in liver cancer tissues and cells. CAMK4 was also found to inhibit liver cells proliferation, migration and invasion, and promote apoptosis. CAMK4 might exert an antitumor effect by inhibiting the activation of mitogen-activated protein kinase (MARK). MiR-129-5p was a tumor suppressor with low expression in liver cancer tissues and cells. CAMK4, which is a direct target gene of miR-129-5p, could inhibit tumor by inhibiting the activation of MAPK signaling pathway.