Studying the Molecular Mechanisms of Ebola Virus with in situ Structural Biology.
Studying the Molecular Mechanisms of Ebola Virus with in situ Structural Biology.
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用原位结构生物学研究埃博拉病毒的分子机制。
DOI：
10.1093/micmic/ozad067.446
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Wan,William
中科院分区：
文献类型：
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作者：
Wan,William
埃博拉病毒是撒哈拉以南非洲的地方病，可引起严重的出血热，病死率为25- 90%。自1976年发现以来，埃博拉疫情的发生频率越来越高，导致近30起疫情，最近一次于2023年1月结束。2013年至2016年最大和最致命的疫情导致远至欧洲和美国的病例，表明埃博拉病毒有可能传播到地理上遥远的地方。因此，埃博拉病毒被认为是一种新出现的传染病，确定其病理学分子机制的努力特别及时。为了研究驱动埃博拉病毒生命周期的分子机制，我的实验室使用并开发了原位结构生物学方法，以研究宿主和病毒分子在接近天然的环境中，如细胞环境或完整的病毒颗粒。我们的方法包括反向遗传学系统，以模拟埃博拉病毒感染降低生物安全条件下，活细胞显微镜，以阐明宿主-病原体相互作用的时空动力学，和冷冻电子断层扫描，以解决分子的细节。
Ebola virus is endemic to Sub-Saharan Africa and causes severe hemorrhagic fevers with case-fatality rates ranging from 25–90%. Since its discovery in 1976, Ebola outbreaks have been occurring with increasing frequency, resulting in nearly 30 outbreaks, with the latest ending in January 2023. The largest and deadliest outbreak from 2013-2016 resulted in cases as far as Europe and the United States, demonstrating that Ebola virus has the potential to spread to geographically distant locations. As such, Ebola virus is considered an emerging infectious disease, and efforts to determine the molecular mechanisms of its pathology are particularly timely. To study the molecular mechanisms that drive the Ebola life cycle, my lab uses and develops in situ structural biology methods to study host and viral molecules within near-native contexts such as within cellular environments or intact viral particles. Our approaches include reverse genetics systems to model Ebola infections under reduced biosafety conditions, live cell microscopy to elucidate the spatiotemporal dynamics of host-pathogen interactions, and cryo-electron tomography to resolve molecular details.