Oligodendrocyte precursor cells from different brain regions express divergent properties consistent with the differing time courses of myelination in these regions.
Oligodendrocyte precursor cells from different brain regions express divergent properties consistent with the differing time courses of myelination in these regions.
复制标题
来自不同大脑区域的少突胶质前体细胞表达与这些区域髓鞘形成的不同时间进程一致的不同特性。
作者：
J. Power;M. Mayer;Joel W. S. Smith;M. Noble
不同的中枢神经系统区域表现出不同的少突胶质细胞生成和髓鞘生成的时间模式。从不同区域分离的少突胶质细胞-2型星形胶质细胞祖细胞（这里缩写为O-2A/OPCs）的特征表明这些发育模式与每个区域中存在的特定O-2A/OPCs的特性一致。从皮层、视神经和视交叉分离的O-2A/OPCs在自我更新和分化特性上存在显著差异。在视神经衍生的O-2A/OPCs在2天内产生少突胶质细胞的条件下，少突胶质细胞在5天后从视交叉衍生的细胞中产生，并且仅在7-10天后从皮质O-2A/OPCs中产生。这些差异，这似乎是细胞内在的（并可能与细胞内的氧化还原状态），表现在减少克隆产生少突胶质细胞的百分比，并在一个较少的代表性少突胶质细胞在个别克隆。此外，视神经，视交叉和皮质衍生的O-2A/OPCs的甲状腺激素（TH）和睫状神经营养因子（CNTF），少突胶质细胞生成的诱导剂的反应，呈负相关的程度观察到的自我更新的基础分裂条件。我们的研究结果表明，迄今尚未认识到的前体细胞之间的复杂性，被认为是少突胶质细胞的直接祖先，并建议这些不同的人口的属性可能有助于不同的时间过程中的髓鞘在不同的中枢神经系统区域。
Different CNS regions exhibit different temporal patterns of oligodendrocyte generation and myelinogenesis. Characterization of oligodendrocyte-type-2 astrocyte progenitor cells (here abbreviated as O-2A/OPCs) isolated from different regions indicates these developmental patterns are consistent with properties of the specific O-2A/OPCs resident in each region. Marked differences were seen in self-renewal and differentiation characteristics of O-2A/OPCs isolated from cortex, optic nerve and optic chiasm. In conditions where optic nerve-derived O-2A/OPCs generated oligodendrocytes within 2 days, oligodendrocytes arose from chiasm-derived cells after 5 days and from cortical O-2A/OPCs only after 7-10 days. These differences, which appear to be cell-intrinsic (and may be related to intracellular redox state), were manifested both in reduced percentages of clones producing oligodendrocytes and in a lesser representation of oligodendrocytes in individual clones. In addition, responsiveness of optic nerve-, chiasm- and cortex-derived O-2A/OPCs to thyroid hormone (TH) and ciliary neurotrophic factor (CNTF), well-characterized inducers of oligodendrocyte generation, was inversely related to the extent of self-renewal observed in basal division conditions. Our results demonstrate hitherto unrecognized complexities among the precursor cells thought to be the immediate ancestors of oligodendrocytes, and suggest that the properties of these different populations may contribute to the diverse time courses of myelination in different CNS regions.
二氢四甲基玫瑰胺：一种长波长荧光过氧化物酶底物，在体外和模型吞噬细胞中进行了评估。
DOI：
10.1016/0006-291x(91)91576-x
发表时间：
1991
影响因子：
3.1
作者：
Whitaker,JE;Moore,PL;Haugland,RP;Haugland,RP
通讯作者：
Haugland,RP