Effect of Aflatoxin BI upon Phytohemagglutinin-Transformed Human Lymphocytes 1
Effect of Aflatoxin BI upon Phytohemagglutinin-Transformed Human Lymphocytes 1
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黄曲霉毒素BI对植物血凝素转化的人淋巴细胞的影响1
DOI：
10.3181/00379727-134-34954
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发表时间：
1970
影响因子：
--
通讯作者：
T. Cardella
中科院分区：
文献类型：
--
作者：
H. Savel;B. Forsyth;W. Schaeffer;T. Cardella
黄曲霉毒素是由某些黄曲霉菌株产生的一组密切相关的有毒代谢物。自从有报道称家禽中毒事件是由于花生粉受到黄曲霉毒素污染 1 造成的，越来越多的文献涉及黄曲霉毒素及其最丰富、最有效的成分黄曲霉毒素 B1 的毒性 2、致癌 3、4 和致畸 5 效应 6 。据观察，致癌物会抑制免疫机制 7, 8，尤其是导致同种移植物排斥的细胞免疫 9 。 Stutman 10 报道了不同品系小鼠对 3-甲基胆蒽引起的肿瘤发展的敏感性与致癌物的免疫抑制之间的相关性。由于淋巴细胞转化被认为是淋巴细胞或细胞免疫的表现11，因此决定研究黄曲霉毒素B1对植物血凝素和特异性抗原体外转化人外周血淋巴细胞的影响。材料和方法。外周血样本取自佛蒙特州医疗中心医院的患者志愿者，他们没有接受皮质类固醇或镇静剂，而且病情并不严重。静脉穿刺后，按照前述方法建立外周血淋巴细胞培养物 12 。简而言之，通过粘附软玻璃去除大部分多形核白细胞和单核细胞，并反复洗涤富含淋巴细胞的血沉棕黄层以去除自体血浆。最终白细胞浓度为40%～70%小淋巴细胞，106个淋巴细胞/ml悬浮于3ml含20%胎牛血清、50μg/ml链霉素、160单位/ml青霉素的培养基（Minimal Essential Medium-Spinner）中。
The aflatoxins are a group of closely related toxic metabolites produced by certain strains of the mold Aspergillus flavus. Since the report that an outbreak of toxicity in poultry was due to contamination of peanut meal with aflatoxin 1 , there has been a growing literature concerning the toxic 2 , carcinogenic 3 , 4 and teratogenic 5 effects of aflatoxin and its most abundant and potent component, aflatoxin B1 6 . Carcinogens have been observed to depress immune mechanisms 7 , 8 , especially the cellular immunity which is responsible for homograft rejection 9 . Stutman 10 has reported a correlation between the sensitivity of various strains of mice to tumor development with 3-methylocholanthrene and immune depression by the carcinogen. Since lymphocyte transformation is considered to be a manifestation of lymphocyte or cellular immunity 11 , it was decided to investigate the effects of aflatoxin B1 on in vitro transformation of human peripheral blood lymphocytes by phytohemagglutinin and specific antigens. Materials and Methods. Peripheral blood samples were obtained from patient volunteers at the Medical Center Hospital of Vermont who were not receiving corticosteroids or tranquilizers and who were not seriously ill. After the venipuncture, peripheral blood lymphocyte cultures were established by the method which has been described previously 12 . In brief, the bulk of the polymorphonuclear leukocytes and monocytes was removed by adherence to soft glass and the lymphocyte-enriched buffy coat washed repeatedly to remove autologous plasma. The final leukocyte concentration was 40% to 70% small lymphocytes, and 106 lymphocytes/ml were suspended in 3 ml of medium (Minimal Essential Medium-Spinner) containing 20% fetal calf serum, 50 μg/ml of streptomycin, and 160 units/ml of penicillin.