Selected acute phase CSF factors in ischemic stroke: findings and prognostic value
Selected acute phase CSF factors in ischemic stroke: findings and prognostic value
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DOI：
10.1186/1471-2377-11-41
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发表时间：
2011-03-30
期刊：
影响因子：
2.6
通讯作者：
Bornstein, Natan M.
中科院分区：
文献类型：
--
作者：
Beridze, Maia;Sanikidze, Tamar;Bornstein, Natan M.
背景：本研究旨在探讨几种反映缺血性脑损伤严重程度的脑脊液急性期因子的致病作用和预后价值。方法：对95例急性缺血性中风患者进行调查。24小时常规磁共振成像显示缺血区。卒中严重程度采用美国国立卫生研究院卒中评定量表进行评估。用Barthel指数评定1个月的疾病转归。卒中后6小时取脑脊液。采用酶联免疫吸附试验检测脑脊液中促炎症细胞因子和抗炎细胞因子。用电子顺磁共振仪测定一氧化氮和过氧化脂质自由基。用Griess试剂检测脑脊液硝酸盐水平。用SPSS-11.0统计软件进行统计学处理。结果：卒中患者发病6h脑脊液细胞因子水平明显高于对照组。与较轻的中风患者相比，重度中风患者的IL-6含量升高(P<；0.05)。脑脊液一氧化氮电子顺磁共振信号增强。与轻度卒中组相比，重度卒中组脂质过氧化自由基电子顺磁共振信号升高。轻度卒中患者脑脊液硝酸盐水平高于重度卒中组和对照组。第7天初始IL-6含量与缺血灶大小、美国国立卫生研究院卒中评分呈正相关。脑脊液中IL-6和硝酸盐的初始水平对卒中后1个月的功能转归有重要意义。结论：脑脊液中IL-6和硝酸盐的含量可能是缺血性卒中急性期最可靠的预后因素。
Background: Study aimed at investigation of pathogenic role and prognostic value of several selected cerebrospinal fluid acute phase factors that can reflect the severity of ischemic brain damage.Methods: Ninety five acute ischemic stroke patients were investigated. Ischemic region visualized at the twenty fourth hour by conventional Magnetic Resonance Imaging. Stroke severity evaluated by National Institute Health Stroke Scale. One month outcome of disease was assessed by Barthel Index. Cerebrospinal fluid was taken at the sixth hour of stroke onset. CSF pro-and anti-inflammatory cytokines were studied by Enzyme Linked Immunosorbent Assay. Nitric Oxide and Lipoperoxide radical were measured by Electron Paramagnetic Resonance. CSF Nitrate levels were detected using the Griess reagent. Statistics performed by SPSS-11.0.Results: At the sixth hour of stroke onset, cerebrospinal fluid cytokine levels were elevated in patients against controls. Severe stroke patients had increased interleukin-6 content compared to less severe strokes (P < 0.05). Cerebrospinal fluid Electron Paramagnetic Resonance signal of nitric oxide was increased in patients against controls. Severe stroke group had an elevated Electron Paramagnetic Resonance signal of lipoperoxiradical compared to less severe stroke. Cerebrospinal fluid nitrate levels in less severe stroke patients were higher than those for severe stroke and control. Positive correlation was established between the initial interleukin-6 content and ischemic lesion size as well as with National Institute Health Stroke Scale score on the seventh day. Initial interleukin-6 and nitrate levels in cerebrospinal fluid found to be significant for functional outcome of stroke at one month.Conclusion: According to present study the cerebrospinal fluid contents of interleukin-6 and nitrates seem to be the most reliable prognostic factors in acute phase of ischemic stroke.