A coordinated codon-dependent regulation of translation by Elongator
A coordinated codon-dependent regulation of translation by Elongator
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DOI：
10.4161/cc.22689
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发表时间：
2012-12-15
期刊：
影响因子：
4.3
通讯作者：
Hermand, Damien
中科院分区：
文献类型：
--
作者：
Bauer, Fanelie;Hermand, Damien
十多年前，Svejstrup实验室对参与延伸的RNA聚合酶II（PolII）的形式进行了纯化，从而发现了一种相关的多亚基（Elp 1 -6）复合物，命名为“Elongator”。虽然进一步的证据支持最初的概念，即延长参与转录，延长缺乏一些预期的功能的调节器的延长PolII。Bystrom实验室基于遗传解剖的发现，即Elongator是tRNA修饰的关键，并且所有报道的Elongator突变体的表型都被两种tRNA的过表达所抑制，这增加了混乱。在高等真核生物中由Elongator调节的潜在底物和生物过程的范围越来越大，表明该领域的主要挑战是确定Elongator的生物相关功能。我们最近在裂殖酵母中的蛋白质组范围的研究支持由Elongator进行的协调的密码子依赖性翻译调节。在这里，我们提供了额外的分析扩展这一假设芽殖酵母和蠕虫。
More than a decade ago, the purification of the form of the RNA polymerase II (PolII) engaged in elongation led to the discovery of an associated, multi-subunit (Elp1-6) complex named "Elongator" by the Svejstrup lab. Although further evidence supported the original notion that Elongator is involved in transcription, Elongator lacked some of the expected features for a regulator of the elongating PolII. The discovery by the Bystrom lab, based on genetic dissection, that Elongator is pivotal for tRNA modifications, and that all the reported phenotypes of Elongator mutants are suppressed by the overexpression of two tRNAs added to the confusion. The increasing range of both potential substrates and biological processes regulated by Elongator in higher eukaryotes indicates that the major challenge of the field is to determine the biologically relevant function of Elongator. Our recent proteome-wide study in fission yeast supports a coordinated codon-dependent regulation of translation by Elongator. Here we provide additional analyses extending this hypothesis to budding yeast and worm.