Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization.
Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization.
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DOI：
10.1101/gad.248583.114
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发表时间：
2014-09-15
影响因子：
10.5
通讯作者：
Fyodorov DV
中科院分区：
文献类型：
--
作者：
Emelyanov AV;Rabbani J;Mehta M;Vershilova E;Keogh MC;Fyodorov DV
有性生殖动物的雄配子中的核 DNA 被组织为主要由精子特异性鱼精蛋白压缩的精子染色质。受精导致精子染色质重塑，在此期间鱼精蛋白被排出并被组蛋白取代。在这里，叶梅利亚诺夫等人。鉴定果蝇鱼精蛋白伴侣介导鱼精蛋白-DNA 复合物的解离。 TAP/p32 是去除果蝇鱼精蛋白 B 所必需的，而 NAP-1、NLP 和 Nph 在去除鱼精蛋白 A 中共同发挥作用。有性生殖动物雄配子中的核 DNA 被组织为主要由精子特异性鱼精蛋白压缩的精子染色质。受精导致精子染色质重塑，在此期间鱼精蛋白被排出并被组蛋白取代。尽管我们对新生雄性原核中介导核小体组装的因素有了更多的了解，但去除鱼精蛋白的机制仍然很大程度上未知。在这里，我们鉴定了四种果蝇鱼精蛋白伴侣，它们介导鱼精蛋白-DNA 复合物的解离：NAP-1、NLP 和核磷蛋白是先前鉴定的组蛋白伴侣，而 TAP/p32 在染色质代谢中没有已知的功能。我们发现 TAP/p32 是体外去除果蝇鱼精蛋白 B 所必需的，而 NAP-1、NLP 和 Nph 在去除鱼精蛋白 A 中共同发挥作用。 P32 缺失雌性的胚胎在体内表现出雄性原核形成缺陷。 TAP/p32 与 NAP-1、NLP 和 Nph 类似，有助于体外核小体组装，因此是一种组蛋白伴侣。此外，P32、Nlp 和 Nph 的突变体表现出合成致死遗传相互作用。总之，我们确定了介导从 DNA 中去除鱼精蛋白的因素，并在一个确定的系统中重建了精子染色质重塑的过程，该过程将鱼精蛋白交换为组蛋白，形成体细胞的基于核小体的染色质特征。
Nuclear DNA in the male gamete of sexually reproducing animals is organized as sperm chromatin compacted primarily by sperm-specific protamines. Fertilization leads to sperm chromatin remodeling, during which protamines are expelled and replaced by histones. Here, Emelyanov et al. identify Drosophila protamine chaperones that mediate the dissociation of protamine–DNA complexes. TAP/p32 is required for the removal of Drosophila protamine B, whereas NAP-1, NLP, and Nph share roles in removal of protamine A. Nuclear DNA in the male gamete of sexually reproducing animals is organized as sperm chromatin compacted primarily by sperm-specific protamines. Fertilization leads to sperm chromatin remodeling, during which protamines are expelled and replaced by histones. Despite our increased understanding of the factors that mediate nucleosome assembly in the nascent male pronucleus, the machinery for protamine removal remains largely unknown. Here we identify four Drosophila protamine chaperones that mediate the dissociation of protamine–DNA complexes: NAP-1, NLP, and nucleophosmin are previously characterized histone chaperones, and TAP/p32 has no known function in chromatin metabolism. We show that TAP/p32 is required for the removal of Drosophila protamine B in vitro, whereas NAP-1, NLP, and Nph share roles in the removal of protamine A. Embryos from P32-null females show defective formation of the male pronucleus in vivo. TAP/p32, similar to NAP-1, NLP, and Nph, facilitates nucleosome assembly in vitro and is therefore a histone chaperone. Furthermore, mutants of P32, Nlp, and Nph exhibit synthetic-lethal genetic interactions. In summary, we identified factors mediating protamine removal from DNA and reconstituted in a defined system the process of sperm chromatin remodeling that exchanges protamines for histones to form the nucleosome-based chromatin characteristic of somatic cells.
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影响因子：
6.8
作者：
GORBALENYA, AE;KOONIN, EV
通讯作者：
KOONIN, EV
影响因子：
4.8
作者：
Emelyanov, Alexander V.;Konev, Alexander Y.;Fyodorov, Dmitry V.
通讯作者：
Fyodorov, Dmitry V.
DOI：
10.1073/pnas.93.19.10423
发表时间：
1996-09-17
影响因子：
11.1
作者：
Bruni, R;Roizman, B
通讯作者：
Roizman, B
影响因子：
2.1
作者：
Harkness, Troy A. A.
通讯作者：
Harkness, Troy A. A.
影响因子：
2.7
作者：
BELLEN, HJ;GREGORY, BK;KIGER, JA
通讯作者：
KIGER, JA