Quantitative cerebral blood flow with optical coherence tomography.
Quantitative cerebral blood flow with optical coherence tomography.
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DOI：
10.1364/oe.18.002477
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发表时间：
2010-02-01
期刊：
影响因子：
3.8
通讯作者：
Boas DA
中科院分区：
文献类型：
--
作者：
Srinivasan VJ;Sakadzić S;Gorczynska I;Ruvinskaya S;Wu W;Fujimoto JG;Boas DA
脑血流量(CBF)的绝对测量是脑病理生理学研究的重要终点。目前还没有公认的高分辨率的大鼠和小鼠脑血流量的体内纵向监测方法。利用三维多普勒光学相干断层成像和颅窗准备，我们提出了测量大鼠大脑皮质局部脑血流量的方法和算法。为此，我们开发并验证了一个定量统计模型来描述静态组织对速度敏感性的影响。该模型用于设计扫描方案和算法，用于灵敏的3D血流测量和皮质血管成像。我们还介绍了一种绝对流量计算方法，该方法不需要对容器角度有明确的了解。我们发现OCT对大鼠脑血流量绝对值的估计与先前通过放射自显影测量的结果一致，这表明多普勒OCT可以在动物模型中进行绝对血流测量。
Absolute measurements of cerebral blood flow (CBF) are an important endpoint in studies of cerebral pathophysiology. Currently no accepted method exists for in vivo longitudinal monitoring of CBF with high resolution in rats and mice. Using three-dimensional Doppler Optical Coherence Tomography and cranial window preparations, we present methods and algorithms for regional CBF measurements in the rat cortex. Towards this end, we develop and validate a quantitative statistical model to describe the effect of static tissue on velocity sensitivity. This model is used to design scanning protocols and algorithms for sensitive 3D flow measurements and angiography of the cortex. We also introduce a method of absolute flow calculation that does not require explicit knowledge of vessel angles. We show that OCT estimates of absolute CBF values in rats agree with prior measures by autoradiography, suggesting that Doppler OCT can perform absolute flow measurements in animal models.