Cross-host evolution of severe acute respiratory syndrome coronavirus in palm civet and human
Cross-host evolution of severe acute respiratory syndrome coronavirus in palm civet and human
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DOI：
10.1073/pnas.0409608102
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发表时间：
2005-02-15
影响因子：
11.1
通讯作者：
Zhao, GP
中科院分区：
文献类型：
--
作者：
Song, HD;Tu, CC;Zhao, GP
2003/2004年在中国广州爆发的人和果子狸的严重急性呼吸综合征冠状病毒的基因组序列几乎相同。系统发育分析表明，在这一新的事件中，病毒从动物到人类的独立入侵。结合所有现有的数据，但不包括单例，我们确定了202个单核苷酸变异。其中，17种仅在果子狸中表现多态性。从不同地理位置收集的果子狸中的非同义/同义核苷酸替换的比率非常高，这表明果子狸中的病毒蛋白也有一个快速进化的过程，就像它们在2002-2003年初流行时在人类宿主中的适应一样。一些关键基因的主要遗传变异，特别是Spike基因，似乎对从动物到人的传播过渡到人到人的传播至关重要，最终导致2002/2003年第一次严重急性呼吸系统综合征爆发。
The genomic sequences of severe acute respiratory syndrome coronaviruses from human and palm civet of the 2003/2004 outbreak in the city of Guangzhou, China, were nearly identical. Phylogenetic analysis suggested an independent viral invasion from animal to human in this new episode. Combining all existing data but excluding singletons, we identified 202 single-nucleotide variations. Among them, 17 are polymorphic in palm civets only. The ratio of nonsynonymous/synonymous nucleotide substitution in palm civets collected I yr apart from different geographic locations is very high, suggesting a rapid evolving process of viral proteins in civet as well, much like their adaptation in the human host in the early 2002-2003 epidemic. Major genetic variations in some critical genes, particularly the Spike gene, seemed essential for the transition from animal-to-human transmission to human-to-human transmission, which eventually caused the first severe acute respiratory syndrome outbreak of 2002/2003.