INCORPORATION OF VARIOUS METAL IONS INTO IN VIVO- AND IN VITRO-PRODUCED MELANIN
INCORPORATION OF VARIOUS METAL IONS INTO IN VIVO- AND IN VITRO-PRODUCED MELANIN
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DOI：
10.2307/3572300
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发表时间：
1967-01-01
影响因子：
3.4
通讯作者：
ATHERTON, DR
中科院分区：
文献类型：
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作者：
BRUENGER, FW;STOVER, BJ;ATHERTON, DR
在从天然来源[C56黑小鼠的B-16黑色素瘤组织]获得的黑色素中发现少量的许多金属离子，并且发现140 Ba和226 Ra存款在含有黑色素的组织中。用高比活度的放射性核素研究了掺入的化学性质的某些方面。合成黑色素从生理缓冲液中摄取的金属离子随着水合离子半径的减小而增加，随着pH值的降低而减少，并且在螯合剂的存在下减少。用H+取代黑色素结合的Ra++证明了反应的可逆性。与合成黑色素和天然黑色素及其蛋白质的比较研究表明，蛋白质在反应中的作用很小。黑色素与弱酸性阳离子交换剂的反应特征和化学结构相似，说明金属离子与黑色素通过离子交换作用发生作用。
Small amounts of a number of metal ions were found in melanins obtained from natural sources [B-16 melanoma tissue of C56 black mouse], and 140Ba and 226Ra were found to deposit in tissues containing melanin. Some aspects of the chemical nature of the incorporation were investigated with radio-nuclides of high specific activity. The uptake of metal ions by synthetic melanin from a physiological buffer increased with decreasing hydrated ionic radius, decreased with decreasing pH, and decreased in the presence of chelating agents. Replacement of melanin-bound Ra++ with H+ demonstrated the reversiblity of the reaction. Comparative studies with synthetic melanin and natural melanin plus its protein showed that the role of the protein in the reactions is small. Both the reaction chracteristics and the chemical structures of melanin and of weak acid cation exchangers are similar, which suggests that metal ions and melanin interact by ion exchange.