MYB94 and MYB96 additively inhibit callus formation via directly repressing LBD29 expression in Arabidopsis thaliana
MYB94 and MYB96 additively inhibit callus formation via directly repressing LBD29 expression in Arabidopsis thaliana
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DOI：
10.1016/j.plantsci.2019.110323
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发表时间：
2020-04-01
期刊：
影响因子：
5.2
通讯作者：
Liu, Zhenhua
中科院分区：
文献类型：
--
作者：
Dai, Xuehuan;Liu, Na;Liu, Zhenhua
植物体细胞在离体培养过程中可以进行重编程。愈伤组织的诱导是植物再生的第一步。近年来的研究表明，生长素诱导的多器官愈伤组织的形成是通过根发育途径从中柱鞘或中柱鞘样细胞开始的。然而，愈伤组织形成的分子控制在很大程度上是未知的。本研究发现，两个MYB转录因子MYB94和MYB96在生长素诱导的拟南芥愈伤组织形成中起负作用。MYB94和MYB96在新形成的愈伤组织中表达。myb96、myb94和myb94 myb96产生的愈伤组织比WT多，其中myb94 myb96产生的愈伤组织最多。MYB94和MYB96通过直接结合基因启动子抑制侧边器官边界结构域29 （LBD29）的表达。LBD29功能缺失部分挽救了myb94 myb96的愈伤组织形成缺陷。我们的研究发现MYB94和MYB96是愈伤组织形成的重要抑制因子，MYB94/96- lbd29是与ARF7/19-LBDs途径平行调控愈伤组织形成的新途径。
Plant somatic cells can be reprogrammed during in vitro culture. Callus induction is the initial step of a typical plant regeneration system. Recent studies showed that auxin-induced callus formation in multiple organs occurs from the pericycle or pericycle-like cells via a root developmental pathway. However, the molecular control of callus formation is largely unknown. Here, two MYB transcription factors, MYB94 and MYB96, were shown to play negative roles in auxin-induced callus formation in Arabidopsis. MYB94 and MYB96 were expressed in the newly formed callus. myb96, myb94, and myb94 myb96 generated more calli than the WT, with myb94 myb96 producing the most. MYB94 and MYB96 repressed expression of LATERAL ORGAN BOUNDARIES-DOMAIN 29 (LBD29) via directly binding to the gene's promoter. The loss of function of LBD29 partly rescued the callus formation defect of myb94 myb96. Our findings found MYB94 and MYB96 to be important repressors of callus formation and MYB94/96-LBD29 as a new regulatory pathway acting in parallel with ARF7/19-LBDs' pathway to modulate in vitro callus formation.