An efficient strategy for assignment of cross-peaks in 3D heteronuclear NOESY experiments
An efficient strategy for assignment of cross-peaks in 3D heteronuclear NOESY experiments
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DOI：
10.1023/a:1008367912535
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发表时间：
1999-10-01
影响因子：
2.7
通讯作者：
Kessler, H
中科院分区：
文献类型：
--
作者：
Diercks, T;Coles, M;Kessler, H
问题是如何最大的结构NOE数据的双标记蛋白可以获得最小的NOE实验集。报道了两个3D-NOESY光谱，它们与其他常用的光谱相一致，为NOE分配提供了一种方便的策略。3D CNH-NOESY和3D NCH-NOESY提供酰胺质子和碳结合质子之间的NOE连接，并构成正交异核滤波器，消除对角信号，显著提高光谱质量。在光谱中记录了两个不同的异核化学位移维数，从而利用了异核的额外色散，大大简化了分配。
The question is addressed of how maximal structural NOE data on double labelled proteins can be acquired with a minimal set of NOESY experiments. Two 3D-NOESY spectra are reported which, in concert with other commonly used spectra, provide a convenient strategy for NOE assignment. The 3D CNH-NOESY and 3D NCH-NOESY provide NOE connectivities between amide protons and carbon-bound protons and constitute orthogonal heteronuclear filters which eliminate diagonal signals, considerably improving spectral quality. Two different heteronuclear chemical shift dimensions are recorded in the spectra, thus exploiting the extra dispersion of the heteronucleus and considerably simplifying assignment.