The PmrA/PmrB regulatory system controls the expression of the wzzfepE gene involved in the O-antigen synthesis of Salmonella enterica serovar Typhimurium
The PmrA/PmrB regulatory system controls the expression of the wzzfepE gene involved in the O-antigen synthesis of Salmonella enterica serovar Typhimurium
复制标题
DOI：
10.1099/mic.0.050088-0
复制
发表时间：
2011-09-01
期刊：
影响因子：
2.8
通讯作者：
Delgado, Monica A.
中科院分区：
文献类型：
--
作者：
de las Mercedes Pescaretti, Maria;Lopez, Fabian E.;Delgado, Monica A.
鼠伤寒沙门氏菌O抗原的聚合度受WZZ(St)和WZZ(FepE)基因产物的控制。在本研究中，我们研究了PmrA/PmrB调控系统在WZZ(FepE)、E转录中的作用。我们报道了PmrA调节子与特定启动子位点的直接结合诱导了WZZ(FepE)基因的表达。这种效应增加了超长(VL)O抗原的数量，这是沙门氏菌对血清人类补体和多粘菌素B产生抵抗力所必需的，也是细菌在巨噬细胞内复制所必需的。这里得到的结果突出了WZZ(FepE)和WZZ(St)之间的功能差异，尽管这两种蛋白的基因都是以PmrA依赖的方式调节的。
The degree of polymerization of O-antigen from Salmonella enterica serovar Typhimurium is controlled by the products of the wzz(st) and wzz(fepE) genes. In the present study we investigated the role of the PmrA/PmrB regulatory system in wzz(fepE),E transcription. We report that the direct binding of the PmrA regulator to a specific promoter site induces the expression of the wzz(fepE) gene. This effect increases the amount of very long (VL) O-antigen, which is required for the resistance of Salmonella to serum human complement and polymyxin B, and for the replication of the bacteria within macrophages. The results obtained here highlight functional differences between Wzz(fepE) and Wzz(st), although the genes for both proteins are regulated in a PmrA-dependent way.