PREVENTION BY GRANULOCYTE DEPLETION OF INCREASED VASCULAR-PERMEABILITY OF SHEEP LUNG FOLLOWING ENDOTOXEMIA
PREVENTION BY GRANULOCYTE DEPLETION OF INCREASED VASCULAR-PERMEABILITY OF SHEEP LUNG FOLLOWING ENDOTOXEMIA
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DOI：
10.1172/jci110371
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发表时间：
1981-01-01
影响因子：
15.9
通讯作者：
BRIGHAM, KL
中科院分区：
文献类型：
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作者：
HEFLIN, AC;BRIGHAM, KL
为了了解循环中的粒细胞是否是肺血管对内毒素(大肠杆菌)反应所必需的，用羟基尿素去除粒细胞前后的内毒素反应在慢性仪器化绵羊中进行了测定。去除粒细胞并不影响内毒素所致的肺动脉高压(平均肺动脉压峰值=38。+-)。耗尽前2厘米H2O和42.+-。2耗竭后，P=NS[无显著意义]。在粒细胞减少的动物中，肺淋巴流量在内毒素后的晚期增加明显较低，这反映在肺淋巴流量(耗竭前的平均稳态淋巴流量=30.6±-)。2.0SEm l/h，粒细胞去除后平均稳态淋巴流量=15.4±-。1.0；P<；0.01)，尽管去粒细胞前后的晚期肺血管压相似。肺淋巴蛋白清除量(淋巴流量。淋巴/血浆蛋白浓度)在粒细胞耗竭后也显著降低(耗竭前平均稳态=21.4.+-)。1.4SE m l/h；耗竭后=10.4.+-。1.0；P<；0.01)。因此，循环中的粒细胞似乎是内毒素后肺血管对液体和蛋白质的渗透性增加所必需的。内毒素对绵羊的肺血管升压作用不依赖于粒细胞。
To see whether circulating granulocytes are necessary for the lung vascular reaction to endotoxin (Escherichia coli), the endotoxin response was measured in chronically instrumented sheep before and after granulocyte depletion with hydroxyurea. Granulocyte depletion did not affect the pulmonary hypertension caused by endotoxin (peak mean pulmonary artery pressures = 38 .+-. 2 cm H2O before depletion and 42 .+-. 2 after depletion, P = NS [nonsignificant]). The late phase increase in lung lymph flow after endotoxin was significantly lower in the granulocytopenic animals as reflected by lung lymph flow (mean steady state lymph flow before depletion = 30.6 .+-. 2.0 SE ml/h; mean steady state lymph flow after granulocyte depletion = 15.4 .+-. 1.0; P < 0.01) even though late phase pulmonary vascular pressures were similar before and after granulocyte depletion. Lung lymph protein clearance (lymph flow .times. lymph/plasma protein concentration) was also significantly lower after granulocyte depletion (mean steady state before depletion = 21.4 .+-. 1.4 SE ml/h; and after depletion = 10.4 .+-. 1.0; P < 0.01). Thus, circulating granulocytes are seemingly necessary for the development of increased lung vascular permeability to fluid and protein following endotoxin. The pulmonary vasopressor effects of endotoxin in sheep are independent of granulocytes.