SURFACE-ASSOCIATED HOST PROTEINS ON VIRULENT TREPONEMA-PALLIDUM
SURFACE-ASSOCIATED HOST PROTEINS ON VIRULENT TREPONEMA-PALLIDUM
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DOI：
10.1128/iai.26.3.1048-1056.1979
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发表时间：
1979-01-01
影响因子：
3.1
通讯作者：
BASEMAN, JB
中科院分区：
文献类型：
--
作者：
ALDERETE, JF;BASEMAN, JB
在强毒T.通过十二烷基硫酸钠凝胶电泳检测苍白球。松散结合的血清蛋白可以通过在无蛋白的培养基中反复洗涤来去除。洗T。苍白球保留了从兔血清和碘化兔或人白蛋白中再吸附许多宿主蛋白的能力。通过免疫吸附技术，用携带蛋白A的葡萄球菌在洗涤的密螺旋体的外包膜上鉴定了各种密切相关的宿主血清蛋白，包括白蛋白、α 2-巨球蛋白、转铁蛋白、血浆铜蓝蛋白、免疫球蛋白[IG] G、IgM和C3 [补体成分3]。透明质酸酶处理没有从洗涤过的密螺旋体表面去除紧密结合的宿主蛋白;胰蛋白酶处理导致凝集水平降低。胰蛋白酶处理的密螺旋体的电泳模式表明，密螺旋体蛋白和吸附的宿主蛋白被蛋白酶消化同时释放。涉及α 2-巨球蛋白和转铁蛋白的再获得研究表明，在密螺旋体外包膜上存在血清蛋白的非竞争性结合位点。T.从个体兔子的梅毒螺旋体制备物中掺入的[35 S]甲硫氨酸、与抗血清的凝集程度和通过胰蛋白酶处理去除与之紧密相关的宿主蛋白所需的时间长度表明了密螺旋体群体之间的生物学变异性。
A surface coat of host serum proteins was detected on virulent T. pallidum by sodium dodecyl sulfate-gel electrophoresis. The loosely associated serum proteins could be removed by repeated washings in a protein-free medium. Washed T. pallidum retained the ability to readsorb numerous host proteins from rabbit serum and iodinated rabbit or human albumin. Various avidly associated host serum proteins including albumin, .alpha.2-macroglobulin, transferrin, ceruloplasmin, immunoglobulin [Ig] G, IgM and C3 [complement component 3] were identified on the outer envelope of washed treponemes by an immunoadsorbent technique with protein A-bearing Staphylococcus. Hyaluronidase treatment did not remove the avidly associated host proteins from the surface of washed treponemes; trypsin treatment resulted in decreased agglutination levels. Electrophoretic patterns of trypsin-treated treponemes showed that treponemal proteins and adsorbed host proteins were released concurrently by protease digestion. Reacquisition studies involving .alpha.2-macroglobulin and transferrin suggested the presence of non-competitive binding sites for serum proteins on the treponemal outer envelope. Differences among the T. pallidum preparations from individual rabbits with respect to incorporation of [35S]methionine, extent of agglutination with antisera and length of time required for removal of avidly associated host proteins by trypsin treatment indicated biological variability among the treponemal populations.