Total Internal Reflection Fluorescence Dynamic Anisotropy of Sulforhodamine 101 at a Liquid/Liquid Interface: Rotational Reorientation Times and Interfacial Structures
Total Internal Reflection Fluorescence Dynamic Anisotropy of Sulforhodamine 101 at a Liquid/Liquid Interface: Rotational Reorientation Times and Interfacial Structures
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Sulforhodamine 101 在液/液界面的全内反射荧光动态各向异性：旋转重定向时间和界面结构
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
N. Kitamura
中科院分区：
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作者：
S. Ishizaka;K. Nakatani;and Satoshi Habuchi;N. Kitamura
采用时间分辨全内反射（TIR）荧光法研究了磺酰罗丹明101（SR 101）在水/邻苯二甲酸酯（PE、邻苯二甲酸二（2-乙基己基）酯、邻苯二甲酸二正庚酯、邻苯二甲酸二正丁酯、邻苯二甲酸二正乙酯）界面上的动态各向异性。TIR荧光动力学的魔角依赖性揭示了SR 101在水/PE界面处的旋转重取向被限制在界面的X-Y平面（面内）中。结果表明，相对于SR 101（1010 A）的分子大小，界面是尖锐的。SR 101在界面处的面内旋转重取向显示出两个时间常数（τrot）。快组分（τ 1 rot）与水溶液中的相似，与PE的性质无关，而慢组分（τ 2 rot）受PE粘度的影响，但不直接受宏观粘度的影响。根据染料在聚乙烯表面的不同吸附模式，解释了SR 101在水/PE界面上的两个旋转重取向时间。
The dynamic anisotropy of Sulforhodamine 101 (SR101) at water/phthalate ester (PE, bis(2-ethylhexyl) phthalate, di-n-heptyl phthalate, di-n-butyl phthalate, or di-n-ethyl phthalate) interfaces was studied by using time-resolved total internal reflection (TIR) fluorometry. A magic-angle dependence of the TIR fluorescence dynamics revealed that rotational reorientation of SR101 at the water/PE interface was restricted in the X−Y plane (in-plane) of the interface. The results indicated that the interface was sharp with respect to the molecular size of SR101 (∼10 A). In-plane rotational reorientation of SR101 at the interface showed two time constants (τrot). The fast component (τ1rot) was similar to that in water irrespective of the nature of PE, while the slow one (τ2rot) was affected by the viscosity of PE but not directly by the macroscopic viscosity. The two rotational reorientation times of SR101 characteristic to the water/PE interface were explained in terms of different adsorption modes of the dye on...