A pure line derived from a self-compatible Chrysanthemum seticuspe mutant as a model strain in the genus Chrysanthemum
A pure line derived from a self-compatible Chrysanthemum seticuspe mutant as a model strain in the genus Chrysanthemum
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DOI：
10.1016/j.plantsci.2019.110174
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发表时间：
2019-10-01
期刊：
影响因子：
5.2
通讯作者：
Kusaba, Makoto
中科院分区：
文献类型：
--
作者：
Nakano, Michiharu;Taniguchi, Kenji;Kusaba, Makoto
菊科是被子植物中最大的科，约有 24,000 种。菊科的分子遗传学研究对于了解植物多样性至关重要。菊花是菊科植物中最重要的工业观赏植物。大多数菊花品种都是同源六倍体且自交不亲和。这些特性是桑叶的遗传分析和现代育种的主要障碍。此外，基因组的高度异质性使分子生物学分析变得复杂。在这项研究中，我们开发了菊花属的模型菌株。 C. seticuspe 是一种二倍体物种，与 C. morifolium 具有相似的开花特性和形态，并且可以进行农杆菌介导的转化。我们分离出一个天然自交亲和突变体C. seticuspe，并通过反复自交和选择建立了纯系。由此产生的菌株被命名为 Gojo-0，由于其基因组的简单性和同质性，有利于遗传分析，包括自然突变体和诱导突变体的分离，并促进分子生物学分析，包括全基因组测序。与菊花物种的种间杂交是可能的，从而能够对自然种间变异进行分子遗传学分析。以Gojo-0为平台积累研究成果和资源，有望推动菊属分子遗传学研究和菊花品种遗传改良。
Asteraceae is the largest family of angiosperms, comprising approximately 24,000 species. Molecular genetic studies of Asteraceae are essential for understanding plant diversity. Chrysanthemum morifolium is the most industrially important ornamental species in Asteraceae. Most cultivars of C. morifolium are autohexaploid and self-incompatible. These properties are major obstacles to the genetic analysis and modern breeding of C. morifolium. Furthermore, high genome heterogeneity complicates molecular biological analyses. In this study, we developed a model strain in the genus Chrysanthemum. C. seticuspe is a diploid species with a similar flowering property and morphology to C. morifolium and can be subjected to Agrobacterium-mediated transformation. We isolated a natural self-compatible mutant of C. seticuspe and established a pure line through repeated selfing and selection. The resultant strain, named Gojo-0, was favorable for genetic analyses, including isolation of natural and induced mutants, and facilitated molecular biological analysis, including whole genome sequencing, owing to the simplicity and homogeneity of its genome. Interspecific hybridization with Chrysanthemum species was possible, enabling molecular genetic analysis of natural interspecific variations. The accumulation of research results and resources using Gojo-0 as a platform is expected to promote molecular genetic studies on the genus Chrysanthemum and the genetic improvement of chrysanthemum cultivars.