Functional domains of the poliovirus receptor.
Functional domains of the poliovirus receptor.
复制标题
脊髓灰质炎病毒受体的功能域。
DOI：
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发表时间：
1991
影响因子：
11.1
通讯作者：
Akio Nomoto
中科院分区：
文献类型：
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作者：
Satoshi Koike;I. Ise;Akio Nomoto
构建了人类脊髓灰质炎病毒受体的许多突变体 cDNA，以鉴定该分子作为受体的重要区域。所有携带编码整个 N 端免疫球蛋白样结构域（结构域 I）的序列的突变 cDNA 都会赋予小鼠 L 细胞脊髓灰质炎病毒的许可性，但缺乏结构域 I 序列的突变 cDNA 则不会。允许脊髓灰质炎病毒的转化体能够结合该病毒，并且也被单克隆抗体D171识别，该抗体与脊髓灰质炎病毒竞争细胞受体。这些结果强烈表明脊髓灰质炎病毒结合位点位于受体的结构域 I 中。还构建了编码胞内肽的序列的突变体cDNA并在小鼠L细胞中表达。这些细胞对脊髓灰质炎病毒的敏感性表明，整个推定的细胞质结构域对于病毒感染并不是必需的。因此，该分子的细胞质结构域似乎在脊髓灰质炎病毒的渗透中不起作用。
A number of mutant cDNAs of the human poliovirus receptor were constructed to identify essential regions of the molecule as the receptor. All mutant cDNAs carrying the sequence coding for the entire N-terminal immunoglobulin-like domain (domain I) confer permissiveness for poliovirus to mouse L cells, but a mutant cDNA lacking the sequence for domain I does not. The transformants permissive for poliovirus were able to bind the virus and were also recognized by monoclonal antibody D171, which competes with poliovirus for the cellular receptor. These results strongly suggest that the poliovirus binding site resides in domain I of the receptor. Mutant cDNAs for the sequence encoding the intracellular peptide were also constructed and expressed in mouse L cells. Susceptibility of these cells to poliovirus revealed that the entire putative cytoplasmic domain is not essential for virus infection. Thus, the cytoplasmic domain of the molecule appears not to play a role in the penetration of poliovirus.