Catalytic behavior and detoxifying ability of an atypical homotrimeric laccase from the thermophilic strain Scytalidium thermophilum on selected azo and triarylmethane dyes
Catalytic behavior and detoxifying ability of an atypical homotrimeric laccase from the thermophilic strain Scytalidium thermophilum on selected azo and triarylmethane dyes
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DOI：
10.1016/j.molcatb.2012.03.017
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发表时间：
2012-07-01
影响因子：
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通讯作者：
Sayadi, Sarni
中科院分区：
文献类型：
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作者：
Ben Younes, Sonia;Bouallagui, Zouhaier;Sayadi, Sarni
研究了漆酶对偶氮染料刚果红和3种三芳基甲烷染料孔雀石绿、溴甲酚绿和酚红的脱色解毒作用。本文研究了pH、温度、离子强度和初始染料浓度等不同的理化参数，以实现嗜热镰刀菌漆酶对染料的最大脱色效果。在0.1 U/ml漆酶存在下，当初始染料浓度为50 mg/l时，酶的脱色率从46%提高到99%。在优化条件下，酶的K-m值为0.25；3.31;苯酚红、刚果红、孔雀石绿和溴甲酚绿分别为5.61和172.1 mM。其中溴甲酚绿在浓度为10000 mg/l时脱色效果最好，6 h内脱色效果达到83%。细胞毒性和微毒性评价表明，最终降解产物的毒性有所降低。(C) 2012 Elsevier B.V.版权所有
Laccase catalyzed decolorization and detoxification was investigated for the azo dye Congo Red and three triarylmethane dyes: Malachite Green, Bromo-Cresol Green and Phenol Red, commonly found in textile industry effluents. In the present work, we studied various physico-chemical parameters such as pH, temperature, ionic strength and initial dye concentration to achieve maximum dye decolorization by Scytalidium thermophilum laccase. The enzyme efficiently decolorized dyes from 46% to 99% at 50 mg/l of initial dye concentration in the presence of 0.1 U/ml of laccase. Under optimized conditions, K-m values of the enzyme were 0.25; 3.31; 5.61 and 172.1 mM toward Phenol Red, Congo Red, Malachite Green and Bromo-Cresol Green, respectively. Among these four dyes, Bromo-Cresol Green at a concentration of 10,000 mg/l showed the best decolorization reaching 83% within 6 h. Cytotoxicity and microtoxicity evaluation demonstrated a decreased toxicity of the final degraded products. (C) 2012 Elsevier B.V. All rights reserved.