Expression of the galanin receptor subtype Gal-R2 mRNA in the rat hypothalamus
Expression of the galanin receptor subtype Gal-R2 mRNA in the rat hypothalamus
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DOI：
10.1016/s0891-0618(99)00011-3
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发表时间：
1999-06-01
影响因子：
2.8
通讯作者：
Beauvillain, JC
中科院分区：
文献类型：
--
作者：
Mitchell, V;Bouret, S;Beauvillain, JC
用大鼠S-35核糖核酸全长探针，用原位杂交法观察甘丙肽受体亚型2(GAL-R2)基因在大鼠下丘脑的分布。Gal-R2受体mRNA表达细胞在下丘脑大部分核团和区域呈中到高水平表达。标记在明确的解剖核内观察到：视前核、视交叉上核、脑室周核、室旁核、弓形核、背内侧核、乳头状核。视上核和腹内侧核几乎没有标记。在垂体内也可见散在的标记细胞。Gal-R2mRNA表达细胞的这种分布与甘丙素结合位点的研究很好地一致。与甘丙肽受体亚型1(Gal-R1型)的分布相比，我们的结果表明Gal-R2型在视前区、弓状核和背内侧核等部分区域存在显著重叠，但分布不同。根据甘丙素受体在神经内分泌调节和摄食行为中的作用，讨论了这些结果的功能含义。(C)1999 Elsevier Science B.V.保留所有权利。
The distribution of galanin receptor subtype 2 (Gal-R2) mRNA-expressing cells was examined by in situ hybridization in the rat hypothalamus using a full-length rat S-35-riboprobe. Gal-R2 receptor mRNA-expressing cells were found at moderate to high levels of expression in most nuclei and regions of hypothalamus. The labeling was observed within well-defined anatomical nuclei: preoptic, suprachiasmatic, periventricular, paraventricular, arcuate, dorsomedial, mammillary nuclei. The supraoptic and ventromedial nuclei were almost devoid of labeling. Some scattered labeled cells were also observed in the pituitary. This distribution of Gal-R2 mRNA-expressing cells corresponds well with that of galanin binding sites studies. As compared to the distribution of the galanin receptor subtype 1 (Gal-R1), our results indicate that the Gal-R2 type is differentially distributed, although a significant overlap exists in some regions such the preoptic area, arcuate and dorsomedial nuclei. The functional implications of these results are discussed in light of the role of galanin receptors plays in neuroendocrine regulation and feeding behavior. (C) 1999 Elsevier Science B.V. All rights reserved.