Evolution of melanocortin receptors in teleost fish: The melanocortin type I receptor
Evolution of melanocortin receptors in teleost fish: The melanocortin type I receptor
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DOI：
10.1016/j.gene.2007.07.005
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发表时间：
2007-10-15
期刊：
影响因子：
3.5
通讯作者：
Volff, Jean-Nicolas
中科院分区：
文献类型：
--
作者：
Selz, Yvonne;Braasch, Ingo;Volff, Jean-Nicolas
I 型黑皮质素受体 (Mc1r) 属于参与脊椎动物各种生理过程的 G 蛋白偶联受体家族。黑皮质素。 Mcr天然激动剂是垂体肽激素，包括促肾上腺皮质激素和黑素细胞刺激激素。在哺乳动物和鸟类中，Mc1r 参与色素沉着并在黑色素细胞和黑色素瘤中表达。 Mc1r 的激活导致真黑色素的产生以及表皮中黑色素细胞的增殖和存活。在这里，我们报告了来自三种主要鱼类模型（斑马鱼斑马鱼、青鳉Oryzias latipes 和扁鱼剑尾鱼）的 mc1r 分子和进化分析。与其他一些黑皮质素受体基因相比，mc1r 在不同的鱼类物种中作为单拷贝基因被保留。在鸭嘴鱼和青鳉的所有测试器官中都检测到了它的表达，但在斑马鱼中仅限于眼睛、皮肤、大脑和睾丸，这可能反映了皮外黑色素细胞分布的差异。 mc1r 基因被发现在胚胎发生过程中表达。以及与人类肿瘤类似的剑尾混合黑色素瘤。鱼类和哺乳动物 Mc1r 之间的蛋白质序列比较揭示了进化和功能分析之间的显着一致性，以识别对受体功能至关重要的残基和区域。 (c) 2007 Elsevier B.V. 保留所有权利。
The melanocortin type I receptor (Mc1r) belongs to a family of G-protein-coupled receptors involved in various physiological processes in vertebrates. Melanocortins. the Mcr natural agonists, are pituitary peptide hormones including adrenocorticutropin and melanocyte-stimulating hormones. In mammals and birds, Mc1r is involved in pigmentation and expressed in melanocytes and melanoma. Activation of Mc1r leads to eumelanin production as well as to proliferation and survival of melanocytes in the epidermis. Here we report the molecular and evolutionary analysis of mc1r from three major fish models, the zebrafish Danio rerio, the medaka Oryzias latipes and the platyfish Xiphophorus maculatus. In contrast to some other melanocortin receptor genes, mc1r has been conserved as a single copy gene in divergent fish species. Its expression was detected in all organs tested in platyfish and medaka but was restricted to eyes, skin, brain and testis in zebrafish, this possibly reflecting differences in the distribution of extracutaneous melanophores. The mc1r gene was found to be expressed during embryogenesis. as well as in Xiphophorus hybrid melanoma, similar to human tumours. Protein sequence comparison between fish and mammalian Mc1r revealed a remarkable concordance between evolutionary and functional analyses for the identification of residues and regions critical for receptor function. (c) 2007 Elsevier B.V. All rights reserved.