VESICLES OF VARIABLE SIZES PRODUCED BY A RAPID EXTRUSION PROCEDURE
VESICLES OF VARIABLE SIZES PRODUCED BY A RAPID EXTRUSION PROCEDURE
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DOI：
10.1016/0005-2736(86)90302-0
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发表时间：
1986-06-13
期刊：
影响因子：
--
通讯作者：
CULLIS, PR
中科院分区：
文献类型：
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作者：
MAYER, LD;HOPE, MJ;CULLIS, PR
来自该实验室的先前研究已经表明，通过将多层囊泡挤出通过孔径为100 nm的聚碳酸酯过滤器可以有效地产生大的单层囊泡（Hope，M. J.，Bally，M.B.，韦伯，G.和Cullis，P.R.（1985）Biochim. Biophys. Acta 812，55-65）。在这项工作中，它表明，类似的程序可以用于生产均匀大小的单层或多层囊泡，通过利用过滤器的孔径范围从30至400 nm。这些囊泡的单层性和捕获效率可以通过在挤出之前冷冻和解冻多层囊泡来显著增强。当使用非常高的脂质浓度（400 mg/ml）时，该程序特别适用，其中通过100和400 nm过滤器挤出冷冻和解冻的多层囊泡分别导致56%和80%的捕获效率。冷冻断裂电子显微镜显示，在这些脂质浓度下产生的囊泡具有尺寸分布和多层性质的程度相比，在较低的脂质水平下产生的系统。这些结果表明，冻融和挤出过程是用于生产可变尺寸和高捕获效率的囊泡的选择技术。
Previous studies from this laboratory have shown that large unilamellar vesicles can be efficiently produced by extrusion of multilamellar vesicles through polycarbonate filters with a pore size of 100 nm (Hope, M.J., Bally, M.B., Webb, G. and Cullis, P.R. (1985) Biochim. Biophys. Acta 812, 55-65). In this work it is shown that similar procedures can be employed for the production of homogeneously sized unilamellar or plurilamellar vesicles by utilizing filters with pore sizes ranging from 30 to 400 nm. The unilamellarity and trapping efficiencies of these vesicles can be significantly enhanced by freezing and thawing the multilamellar vesicles prior to extrusion. This procedure is particularly applicable when very high lipid concentrations (400 mg/ml) are used, where extrusion of the frozen and thawed multilamellar vesicles through 100 and 400 nm filters results in trapping efficiencies of 56 and 80%, respectively. Freeze-fracture electron microscopy revealed that vesicles produced at these lipid concentrations exhibit size distributions and extent of multilamellar character comparable to systems produced at lower lipid levels. These results indicate that the freeze-thaw and extrusion process is the technique of choice for the production of vesicles of variable sizes and high trapping efficiency.