Indispensable Structure of Solution Additives to Prevent Inactivation of Lysozyme for Heating and Refolding
Indispensable Structure of Solution Additives to Prevent Inactivation of Lysozyme for Heating and Refolding
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DOI：
10.1002/btpr.245
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发表时间：
2009-09-01
影响因子：
2.9
通讯作者：
Shiraki, Kentaro
中科院分区：
文献类型：
--
作者：
Matsuoka, Tsuneyoshi;Hamada, Hiroyuki;Shiraki, Kentaro
本文研究了防止溶菌酶在热处理和复性过程中错误折叠的溶液添加剂。天然溶菌酶的热处理和氧化复性从还原和变性状态的溶菌酶在44种不同的添加剂的存在下的比较揭示了一个不可缺少的化学结构的添加剂是有效的热诱导的错误折叠和复性。对天然溶菌酶的热处理有效的添加剂具有氨基酸部分的主链。具有双酯化和酰胺化羧基的氨基酸比氨基酸本身更有效地防止热诱导的错误折叠。另一方面，对氧化复性有效的添加剂具有胍基或脲基。前一种添加剂阻止了未折叠多肽的主链之间的疏水相互作用，而后一种添加剂增加了芳香族和脂肪族侧链的溶解度。这些数据还支持精氨酸（Arg）和Arg衍生物是两种错误折叠过程的通用添加剂的事实。(C)2009年美国化学工程师学会生物技术。程序，25：1515-1524，2009
This article investigates solution additives that prevent misfolding of lysozyme from heating treatment and during refolding processes. Comparison of heat treatment of native lysozyme and oxidative refolding from the reduced and denatured state of lysozyme in the presence of 44 different additives revealed an indispensable chemical structure for the additives to be effective against heat-induced misfolding and for refolding. The additives effective against heat treatment of native lysozyme possessed a main chain of the amino acid moiety. Amino acids that have esterificated and amidated carboxy groups prevented heat-induced misfoldings more effectively than amino acids themselves. On the other hand, the additives effective against oxidative refolding possessed a guanidium or ureido group. The former additives prevented hydrophobic interaction between the main chains of the unfolded polypeptide, while the latter additives increased the solubility of the aromatic and aliphatic side-chains. These data also support the fact that arginine (Arg) and Arg derivatives are versatile additives for both misfolding processes. (C) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 25: 1515-1524, 2009