Molecular characterization of the conoid complex in Toxoplasma reveals its conservation in all apicomplexans, including Plasmodium species.
Molecular characterization of the conoid complex in Toxoplasma reveals its conservation in all apicomplexans, including Plasmodium species.
复制标题
弓形虫中的锥形复合物的分子特征揭示了其在所有顶复门动物中的保守性，包括疟原虫属物种。
DOI：
10.1371/journal.pbio.3001081
复制
发表时间：
2021-03
期刊：
影响因子：
9.8
通讯作者：
Waller RF
中科院分区：
文献类型：
--
作者：
Koreny L;Zeeshan M;Barylyuk K;Tromer EC;van Hooff JJE;Brady D;Ke H;Chelaghma S;Ferguson DJP;Eme L;Tewari R;Waller RF
顶端复合体是顶端复合体寄生虫入侵的工具，锥体是这个门的一个显著特征。然而，这种锥形被认为在疟原虫物种和Aconoidasida纲的其他成员中严重减少或缺失。以前发现的锥状蛋白相对较少，这使得很难解决这一特征在整个类群中的保守程度，以及它是否真的在一些主要群体中缺失。此外，寄生虫，如疟原虫物种，通过三种入侵形式循环，在这些阶段之间存在差异的锥形的可能性。我们应用空间蛋白质组学和高分辨率显微镜来开发更完整的分子清单，并了解弓形虫模型中圆锥相关蛋白的组织。这些数据揭示了包括疟原虫在内的整个顶复合体中所有锥形亚结构的分子保守性，甚至在近缘的共生动物中也是如此，如Chromera和DinoFlagellates。我们对疟疾模型伯氏疟原虫中几种锥形复合体蛋白的表达和定位进行了标记和观察，揭示了其所有带状体型的相同结构，以及蚊媒的血期裂殖子和动子和子孢子之间存在分子分化的证据。总而言之，我们证明了锥体是一种保守的顶端复合体元件，处于这些高度成功且往往具有破坏性的寄生虫入侵机制的核心。对弓形虫入侵相关锥体结构的蛋白质组学特征表明，这种结构在顶复合体寄生虫中普遍存在，包括疟疾寄生虫疟原虫，而以前人们认为这种结构是不存在的。
The apical complex is the instrument of invasion used by apicomplexan parasites, and the conoid is a conspicuous feature of this apparatus found throughout this phylum. The conoid, however, is believed to be heavily reduced or missing from Plasmodium species and other members of the class Aconoidasida. Relatively few conoid proteins have previously been identified, making it difficult to address how conserved this feature is throughout the phylum, and whether it is genuinely missing from some major groups. Moreover, parasites such as Plasmodium species cycle through 3 invasive forms, and there is the possibility of differential presence of the conoid between these stages. We have applied spatial proteomics and high-resolution microscopy to develop a more complete molecular inventory and understanding of the organisation of conoid-associated proteins in the model apicomplexan Toxoplasma gondii. These data revealed molecular conservation of all conoid substructures throughout Apicomplexa, including Plasmodium, and even in allied Myzozoa such as Chromera and dinoflagellates. We reporter-tagged and observed the expression and location of several conoid complex proteins in the malaria model P. berghei and revealed equivalent structures in all of its zoite forms, as well as evidence of molecular differentiation between blood-stage merozoites and the ookinetes and sporozoites of the mosquito vector. Collectively, we show that the conoid is a conserved apicomplexan element at the heart of the invasion mechanisms of these highly successful and often devastating parasites. Proteomic characterisation of the invasion-related conoid structure in Toxoplasma reveals that this structure is ubiquitous in apicomplexan parasites, including the malaria parasite Plasmodium, where it was previously thought to be absent.
登录
查看更多内容
影响因子：
6.4
作者：
Chen AL;Kim EW;Toh JY;Vashisht AA;Rashoff AQ;Van C;Huang AS;Moon AS;Bell HN;Bentolila LA;Wohlschlegel JA;Bradley PJ
通讯作者：
Bradley PJ
弓形虫 IMC 蛋白的一个新家族在协调寄生虫分裂中表现出层次结构和功能。
影响因子：
6.7
作者：
Beck, Josh R.;Rodriguez-Fernandez, Imilce A.;de Leon, Jessica Cruz;Huynh, My-Hang;Carruthers, Vern B.;Morrissette, Naomi S.;Bradley, Peter J.
通讯作者：
Bradley, Peter J.
影响因子：
16.6
作者：
Christoforou A;Mulvey CM;Breckels LM;Geladaki A;Hurrell T;Hayward PC;Naake T;Gatto L;Viner R;Martinez Arias A;Lilley KS
通讯作者：
Lilley KS
DOI：
10.1016/0035-9203(63)90007-5
发表时间：
1963-01-01
影响因子：
2.2
作者：
GARNHAM, P C;BIRD, R G;BAKER, J R
通讯作者：
BAKER, J R
影响因子：
4.3
作者：
Crook OM;Mulvey CM;Kirk PDW;Lilley KS;Gatto L
通讯作者：
Gatto L