A microfluidic device for dry sample preservation in remote settings.
A microfluidic device for dry sample preservation in remote settings.
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DOI：
10.1039/c3lc50747e
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发表时间：
2013-11-21
期刊：
影响因子：
6.1
通讯作者：
Ismagilov RF
中科院分区：
文献类型：
--
作者：
Begolo S;Shen F;Ismagilov RF
本文描述了一种在室温下干式保存生物标本的微流控装置，该装置结合了化学稳定基质。样本的长期稳定对于远程医疗分析、生物监测和存档至关重要，但目前运输远程获得的样本的范例依赖于昂贵的“冷链”来保存生物标本中的分析物。我们提出了一种替代方法，涉及使用微流体来保存干燥状态下的样品，以及其他人开发的稳定基质，这些稳定基质是基于自然界中发现的自我保护化学物质。我们描述了一种基于SlipChip的设备，它允许受过最低限度培训的用户通过三个简单的步骤来保存样本：将样本放在入口处，关闭盖子，以及滑动设备的一层。该设备自动填充，预加载的干燥剂将样品干燥。随后，可以对标本进行再水化并回收，以便在实验室进行分析。这款设备便携、紧凑、自给自足，因此即使在资源有限的情况下，也可以由未经培训的用户运输和操作。诸如末端填充和顺序填充等特点，与“泵盖”机制相结合，能够精确地量化原始样品的体积，同时避免过度填充。此外，我们还演示了该设备可以与血浆过滤模块集成，并通过测试纯化的RNA溶液的稳定性来验证设备的操作和能力。该平台的这些特征和模块化(便于集成和简化操作)将适用于该应用以外的其他微流控设备。我们预见，随着稳定基质领域的发展，微流控设备将有助于经济高效地促进远程分析和生物监测，同时也为诊断、药物开发和其他医疗领域开辟新的机会。
This paper describes a microfluidic device for dry preservation of biological specimens at room temperature that incorporates chemical stabilization matrices. Long-term stabilization of samples is crucial for remote medical analysis, biosurveillance, and archiving, but the current paradigm for transporting remotely obtained samples relies on the costly “cold chain” to preserve analytes within biospecimens. We propose an alternative approach that involves the use of microfluidics to preserve samples in the dry state with stabilization matrices, developed by others, that are based on self-preservation chemistries found in nature. We describe a SlipChip-based device that allows minimally trained users to preserve samples with the three simple steps of placing a sample at an inlet, closing a lid, and slipping one layer of the device. The device fills automatically, and a pre-loaded desiccant dries the samples. Later, specimens can be rehydrated and recovered for analysis in a laboratory. This device is portable, compact, and self-contained, so it can be transported and operated by untrained users even in limited-resource settings. Features such as dead-end and sequential filling, combined with a “pumping lid” mechanism, enable precise quantification of the original sample’s volume while avoiding overfilling. In addition, we demonstrated that the device can be integrated with a plasma filtration module, and we validated device operations and capabilities by testing the stability of purified RNA solutions. These features and the modularity of this platform (which facilitates integration and simplifies operation) would be applicable to other microfluidic devices beyond this application. We envision that as the field of stabilization matrices develops, microfluidic devices will be useful for cost-effectively facilitating remote analysis and biosurveillance while also opening new opportunities for diagnostics, drug development, and other medical fields.
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