Living material assembly of bacteriogenic protocells
Living material assembly of bacteriogenic protocells
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DOI：
10.1038/s41586-022-05223-w
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发表时间：
2022-09-14
期刊：
影响因子：
64.8
通讯作者：
Mann, Stephen
中科院分区：
文献类型：
--
作者：
Xu, Can;Martin, Nicolas;Mann, Stephen
推进具有高度组织复杂性和多样功能的人工细胞的自发自下而上的构建仍然是生物和非生物物质之间界面的未解决问题（1-4）。在这里，为了解决这一挑战，我们开发了一个活的材料组装过程的基础上捕获和现场处理的空间隔离的细菌菌落内的个别凝聚微滴的膜结合，分子拥挤，和组成，结构和形态复杂的合成细胞的内源性建设。细菌原细胞继承了不同的生物成分，表现出多功能的拟细胞特性，并可以内源性重塑，包括一个空间分区的DNA-组蛋白核样冷凝物，膜化的水空泡和三维网络的F-肌动蛋白原细胞骨架丝。该系统由植入的活大肠杆菌细胞产生的ATP生物化学供能，以产生具有阿米巴样外部形态和综合生命特性的细胞仿生系统。我们的研究结果表明，自下而上的功能protoliving微器件的建设，并为新的合成细胞模块和增强的生活/合成细胞结构的制造提供了机会，在工程合成生物学和生物技术的潜在应用。
Advancing the spontaneous bottom-up construction of artificial cells with high organizational complexity and diverse functionality remains an unresolved issue at the interface between living and non-living matter(1-4). Here, to address this challenge, we developed a living material assembly process based on the capture and on-site processing of spatially segregated bacterial colonies within individual coacervate microdroplets for the endogenous construction of membrane-bounded, molecularly crowded, and compositionally, structurally and morphologically complex synthetic cells. The bacteriogenic protocells inherit diverse biological components, exhibit multifunctional cytomimetic properties and can be endogenously remodelled to include a spatially partitioned DNA-histone nucleus-like condensate, membranized water vacuoles and a three-dimensional network of F-actin proto-cytoskeletal filaments. The ensemble is biochemically energized by ATP production derived from implanted live Escherichia coli cells to produce a cellular bionic system with amoeba-like external morphology and integrated life-like properties. Our results demonstrate a bacteriogenic strategy for the bottom-up construction of functional protoliving microdevices and provide opportunities for the fabrication of new synthetic cell modules and augmented living/synthetic cell constructs with potential applications in engineered synthetic biology and biotechnology.