Biophysical characterization of nanoparticle-endothelial model cell membrane interactions
Biophysical characterization of nanoparticle-endothelial model cell membrane interactions
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DOI：
10.1021/mp700140a
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发表时间：
2008-05-01
影响因子：
4.9
通讯作者：
Labhasetwar, Vinod
中科院分区：
文献类型：
--
作者：
Peetla, Chiranjeevi;Labhasetwar, Vinod
了解纳米颗粒（NPs）与细胞膜的生物物理相互作用对于开发有效的药物递送纳米载体系统至关重要。我们开发了内皮模型细胞膜（EMM），使用脂质和朗缪尔平衡的混合物来研究其与NPs的相互作用。以不同表面化学性质和尺寸的聚苯乙烯NPs为模型纳米材料，以膜表面压力（SP）的变化为参数，监测其与NPs的相互作用。胺化NPs （60 nm）增加SP，普通NPs降低SP，相同大小的羧化NPs没有影响。然而，与表面化学无关，较小的NPs （20 nm）增加了SP，血清不影响其SP效应，而它掩盖了较大（bbb60 nm）的普通和羧化NPs的作用，而不是胺化NPs的作用。由单一磷脂形成的膜与NPs的相互作用模式与EMM的相互作用模式不同，这表明需要使用代表各自感兴趣的细胞/组织的脂质混合物来制作模型膜。通过原子力显微镜和pi-A（表面压力-面积）等温线来确定NP特性对SP的特殊影响，可以根据相互作用是否导致磷脂的缩聚（SP增加）或它们从界面位移到亚相（SP减少），从而导致膜的不稳定来解释。我们得出结论，NP特性显著影响与膜的生物物理相互作用。此外，纳米颗粒与模型膜相互作用的分子机制可以有效地用于优化纳米材料的特性，以用于特定的生物应用。
Understanding the biophysical interactions of nanoparticles (NPs) with cell membranes is critical for developing effective nanocarrier systems for drug delivery applications. We developed an endothelial model cell membrane (EMM) using a mixture of lipids and Langmuir balance to study its interaction with NPs. Polystyrene NPs of different surface chemistry and sizes were used as a model nanomaterial, and changes in the membrane's surface pressure (SP) were used as a parameter to monitor its interactions with NPs. Aminated NPs (60 nm) increased SP, plain NPs reduced it, and carboxylated NPs of the same size had no effect. However, smaller NPs (20 nm) increased SP irrespective of surface chemistry, and serum did not influence their SP effect, whereas it masked the effect of larger (>60 nm) plain and carboxylated but not that of aminated NPs. Membranes formed with a single phospholipid showed a different pattern of interactions with NPs than that with EMM, signifying the need of using a mixture of lipids representing the respective cells/tissue of interest for a model membrane. The particular effect of NP characteristics on SP, determined using atomic force microscopy and,pi-A (surface pressure-area) isotherm, can be explained on the basis of whether the interaction results in condensation of phospholipids (increase in SP) or their displacement from the interface into the subphase (decrease in SP), causing destabilization of the membrane. We conclude that NP characteristics significantly influence biophysical interactions with the membrane. Further, the molecular mechanism(s) of nanoparticle interactions with model membranes can be effectively used for optimizing the characteristics of nanomaterials for particular biological applications.