Effective treatment for suppression of acrylamide formation in fried potato chips using L-asparaginase from Bacillus subtilis.
Effective treatment for suppression of acrylamide formation in fried potato chips using L-asparaginase from Bacillus subtilis.
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DOI：
10.1007/s13205-015-0278-5
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发表时间：
2015-10
期刊：
影响因子：
2.8
通讯作者：
Wakayama M
中科院分区：
文献类型：
--
作者：
Onishi Y;Prihanto AA;Yano S;Takagi K;Umekawa M;Wakayama M
据报，丙烯酰胺是一种潜在致癌物，是由L-天冬酰胺（L-Asn）与食物中的还原糖在加热过程中反应而成，而游离天冬酰胺是丙烯酰胺形成的限制因素。据报，经加热工序制成的马铃薯产品，例如薯片，含有大量丙烯酰胺。为了降低马铃薯中天冬酰胺的含量，研究了用L-天冬酰胺酶处理马铃薯切片的有效条件。通过用枯草芽孢杆菌L-天冬酰胺酶II（BAsnase; 4U/g马铃薯）处理马铃薯切片，马铃薯切片中约40%的L-Asn转化为L-天冬氨酸（L-Asp）。为了使这种酶更有效，在酶处理之前，将切片的马铃薯冷冻-解冻，在90 °C下干燥20分钟，并在减压条件下真空处理10分钟，导致约90%的L-Asn水解为L-Asp。经BAsnase处理的油炸马铃薯片的丙烯酰胺含量降低至未经BAsnase处理的油炸马铃薯片的丙烯酰胺含量的20%以下。在这项研究中检查的处理条件被发现是有效的，以抑制丙烯酰胺的形成油炸薯片。
It has been reported that acrylamide, a potential carcinogen, is formed from the reaction of L-asparagine (L-Asn) and reducing sugars contained in foods during heating processes and free asparagine is a limiting factor for acrylamide formation. It has been reported that potato products such as potato chips, which are made through heating processes, contain high levels of acrylamide. To decrease the amount of L-Asn in potatoes using L-asparaginase, effective treatment conditions of sliced potatoes with the enzyme have been investigated. By treating sliced potatoes with Bacillus subtilis L-asparaginase II (BAsnase; 4 U/g potato), appriximately 40 % of L-Asn in the sliced potatoes was converted into L-aspartic acid (L-Asp). To make this enzyme more effective, prior to enzymatic treatment, sliced potatoes were freeze-thawed, dried at 90 °C for 20 min, and vacuum treated for 10 min under decompressed condition, resulting in the hydrolysis of approximately 90 % of L-Asn to L-Asp. The acrylamide content of BAsnase-treated fried potato chips decreased to below 20 % of that of BAsnase-untreated fried potato chips. Treatment conditions examined in this study were found to be effective to suppress the formation of acrylamide in fried potato chips.