An electrochemical immunosensor to minimize the nonspecific adsorption and to improve sensitivity of protein assays in human serum
An electrochemical immunosensor to minimize the nonspecific adsorption and to improve sensitivity of protein assays in human serum
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DOI：
10.1016/j.bios.2012.05.014
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发表时间：
2012-10-01
影响因子：
12.6
通讯作者：
Trau, Matt
中科院分区：
文献类型：
--
作者：
Shiddiky, Muhammad J. A.;Kithva, Prakash H.;Trau, Matt
描述了一种电化学免疫测定法，其最小化非特异性蛋白质吸附并提高蛋白质组癌症生物标志物的检测灵敏度。我们的技术包括两个新的特点：（i）高密度末端官能化的聚通过表面引发的可逆加成断裂链转移（RAFT）聚合方法从电极表面生长（N-异丙基丙烯酰胺）“刷”层，以使血清蛋白和其它生物分子的非特异性吸附最小化，和（ii）由修饰有CdSe量子点的氧化石墨烯纳米片和针对MSLN的重组单链可变片段组成的信号放大“生物纳米缀合物”，用于“物理地”放大阳极溶出伏安信号。该方法的检测限为ca。血清样品中加标1 pg/mL MSLN（RSD = 4.6%，n=4）。由于这种方法的简单，特异性和敏感性，我们认为它可能会在基于血清的蛋白质诊断中找到潜在的用途。(C)2012爱思唯尔有限公司版权所有。
An electrochemical immunoassay which minimizes nonspecific protein adsorption and improves detection sensitivity of proteomic cancer biomarker is described. Our technique comprises two novel features: (i) a high density terminally functionalized poly(N-isopropyl acrylamide) 'brush' layer is grown by surface initiated reversible addition fragmentation chain transfer (RAFT) polymerization method from the electrode surface in order to minimize nonspecific adsorption of serum proteins and other biomolecules, and (ii) a signal amplifying 'bionanoconjugate' comprised of graphene oxide nanosheets decorated with CdSe quantum dots and recombinant single-chain variable fragments towards MSLN, is used to 'physically' amplify the anodic stripping voltammetric signal. This method enabled a detection limit of ca. 1 pg/mL MSLN (RSD = 4.6%, n=4) spiked in serum samples. Because of the simple, specific and sensitive nature of this methodology, we feel that it may find potential use in serum-based protein diagnostics. (C) 2012 Elsevier B.V. All rights reserved.