2,7-BIS(DIETHYLAMINO)PHENAZOXONIUM CHLORIDE AS A QUANTUM COUNTER FOR EMISSION MEASUREMENTS BETWEEN 240 AND 700 NM
2,7-BIS(DIETHYLAMINO)PHENAZOXONIUM CHLORIDE AS A QUANTUM COUNTER FOR EMISSION MEASUREMENTS BETWEEN 240 AND 700 NM
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DOI：
10.1021/ac00275a039
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发表时间：
1984-01-01
影响因子：
7.4
通讯作者：
HEINZE, J
中科院分区：
文献类型：
--
作者：
KOPF, U;HEINZE, J
苯并唑仑染料Oxazine 170 （2,7 -bls（乙基氨基）-6-甲基- 3,4 -苯并唑仑高氯酸盐）、Nile Blue A （2-amlno-7-（四甲基氨基）- 3,4 -苯并唑仑氯）、Basic Blue 3 （2,7 -bls（二乙基氨基）苯并唑仑氯）以及Cyanine染料HITC（1,1 ', 3,3,3 ', 3'-六亚甲基吲哚基碳氰）已在标准荧光计中作为普遍适用的量子计数器进行了测试。所有染料都可以在长波长范围内用于校正发射和激发光谱，最高可达700 nm。在短波长范围内，只有基蓝3达到220-240 nm的下限，这是由于它的发射强度最大值的有利范围。将潜在量子计数器的性质与罗丹恩b进行了比较，只有基本蓝3在质量上与罗丹恩b相等，有时甚至优于b。原则上，传统测量技术记录的荧光光谱与真实光谱不一致。不同波长相关的仪器参数会改变发射信号的测量强度，从而导致未校正的光谱。虽然这对于分析目的来说是足够的，但在这种情况下，比较光谱和确定量子产率是不可能的。幸运的是，仪器特有的影响可以通过相应的校正来消除
The phenazoxonlum dyes Oxazine 170 (2, 7-bls (ethyl-amino)-6-methyl-3, 4-benzophenazoxonlum perchlorate), Nile Blue A (2-amlno-7-(dlmethylamlno)-3, 4-benzophenazoxonlum chloride), and Basic Blue 3 (2, 7-bls (dlethylamlno) phenazoxonlum chloride) as well as the Cyanine dye HITC (1, 1', 3, 3, 3', 3'-hexamethyllndotrlcarbocyanlne) have been tested as universally applicable quantum counters In standard fluorlmeters. All dyes can be used In the long wavelength range for the correction of emission and excitation spectra up to 700 nm. In the short wavelength range only Basic Blue 3 reaches a lower limit of 220-240 nm on account of the favorable range of the Intensity maximum of Its emission. The properties of the potential quantum counters were compared to those of Rhodamlne B. Only Basic Blue 3 Is qualitatively equal and sometimes even superior to Rhodamlne B.In principle, no fluorescence spectrum recorded with con-ventional measuring techniques is identical with the true spectrum. Different wavelength-dependent instrument pa-rameters alter the measured intensity of the emission signal, which results in a noncorrected spectrum. Although this may be sufficient for analytical purposes, it is impossibleto com-pare spectra and determine quantum yields under these cir-cumstances. Fortunately, the instrument-specific influences can be eliminated with the help of corresponding correction