Microtubule-severing enzymes
Microtubule-severing enzymes
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DOI：
10.1016/j.cub.2022.08.046
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发表时间：
2022-10
期刊：
影响因子：
9.2
通讯作者：
Stephanie L. Sarbanes;E. Zehr;A. Roll-Mecak
中科院分区：
文献类型：
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作者：
Stephanie L. Sarbanes;E. Zehr;A. Roll-Mecak
(A)切断酶spastin和katanin组装成六聚体，其核心具有AAA-ATP酶结构域和柔性臂，该柔性臂招募效应子用于定位和切断活性调节。(B)在AAA-ATP酶核心的中心孔中的互连双螺旋协调微管蛋白尾部，并且被认为拉动它以将微管蛋白从微管中提取出来。切断作用由翻译后修饰顺式调节，或由限制酶可及性的MAP反式调节。
(A) The severing enzymes spastin and katanin assemble into hexamers with AAA-ATPase domains at their core and flexible arms that recruit effectors for localization and severing activity regulation.(B) An interconnected double spiral in the central pore of the AAA-ATPase core coordinates the tubulin tail and is thought to pull on it to extract tubulin out of the microtubule. Severing is regulated in cis by post-translational modifications or in trans by MAPs that limit enzyme accessibility.