Incubation of exogenous fatty acids with lymphocytes. Changes in fatty acid composition and effects on the rotational relaxation time of 1,6-diphenyl-1,3,5-hexatriene.
Incubation of exogenous fatty acids with lymphocytes. Changes in fatty acid composition and effects on the rotational relaxation time of 1,6-diphenyl-1,3,5-hexatriene.
复制标题
外源脂肪酸与淋巴细胞的孵育。
DOI：
10.1021/bi00553a034
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发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
S. M. Johnson
中科院分区：
文献类型：
--
作者：
C. Stubbs;W. M. Tsang;J. Belin;A. D. Smith;S. M. Johnson
用各种与白蛋白结合的长链脂肪酸孵育淋巴细胞。与不饱和脂肪酸孵育20小时，导致中性脂和磷脂被摄取。刀豆蛋白A的加入促进了摄取。不饱和脂肪酸降低了1,6-二苯基-1,3,5-己三烯（DPH）的旋转弛豫时间，而饱和脂肪酸对DPH的影响很小。发现不饱和脂肪酸对旋转松弛时间的影响是由于细胞质中脂滴的形成。当制备不含脂滴的质膜时，无论在膜磷脂中加入亚油酸盐或棕榈酸盐，对旋转松弛时间都没有影响。磷脂脂质体是由细胞的质膜制备的，这些细胞在有和没有外源性亚油酸的情况下培养，无论细胞的历史如何，它们都具有相同的旋转松弛时间。综上所述，虽然外源脂肪酸可以改变淋巴细胞细胞膜的脂肪酸组成，但从对DPH旋转弛豫时间的影响来看，外源脂肪酸对细胞膜磷脂脂肪酸酰基链的有序度影响不大。
Lymphocytes were incubated with various long chain fatty acids bound to albumin. Incubation for 20 h with unsaturated fatty acids resulted in uptake into the neutral lipids and phospholipids. The addition of concanavalin A enhanced the uptake. With the unsaturated fatty acids the rotational relaxation time of 1,6-diphenyl-1,3,5-hexatriene (DPH) was decreased, while with saturated fatty acids there was only a very small effect. The effect on the rotational relaxation times with unsaturated fatty acids was found to be due to the formation of lipid droplets in the cytoplasm. When plasma membrane free of lipid droplets was prepared, there was no effect on the rotational relaxation time despite incorporation of either linoleate or palmitate into the membrane phospholipids. Phospholipid liposomes prepared from plasma membranes obtained from cells which had been cultured with and without exogenous linoleate gave identical rotational relaxation times regardless of the history of the cells from which they were obtained. It is concluded that although the fatty acid composition of lymphocyte plasma membrane can be modified by exogenous fatty acids, there is little effect on the degree of order of the membrane phospholipid fatty acyl chains as monitored by the effects on the rotational relaxation time of DPH.