On the use of 31P NMR for the quantification of hydrosoluble phosphorus-containing compounds in coral host tissues and cultured zooxanthellae.
On the use of 31P NMR for the quantification of hydrosoluble phosphorus-containing compounds in coral host tissues and cultured zooxanthellae.
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DOI：
10.1038/srep21760
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发表时间：
2016-02-23
影响因子：
4.6
通讯作者：
Grover R
中科院分区：
文献类型：
--
作者：
Godinot C;Gaysinski M;Thomas OP;Ferrier-Pagès C;Grover R
利用~（31）P核磁共振（NMR）研究了石珊瑚Stylophora pistillata和养殖虫黄藻（CZ）组织中的含磷化合物。结果表明，CZ中的含磷化合物主要为磷酸酯和磷酸酯。在低磷水平（0.02 μM）下，磷酸盐占CZ中观察到的总磷化合物的19 ± 2%。向CZ培养基中加入5 mM溶解的无机磷（KH 2 PO 4）导致细胞内磷酸盐增加3.1倍，而加入5 mM溶解的有机磷导致磷化合物浓度降低，包括细胞内磷酸盐减少2.5倍。与虫黄嘌呤形成鲜明对比的是，宿主主要含有膦酸盐，并且在较小程度上含有磷酸酯和磷酸盐。两个月的主机饥饿减少磷酸盐含量的2.4倍，而漂白的喂养珊瑚没有修改这个内容。基于31 P NMR分析，这项研究突出了膦酸盐在珊瑚宿主组织组成中的重要性，并说明了磷的可用性对宿主组织和CZ的磷组成的影响，无论是通过喂养的主机和无机磷富集的CZ。
31P Nuclear Magnetic Resonance (NMR) was assessed to investigate the phosphorus-containing compounds present in the tissues of the scleractinian coral Stylophora pistillata as well as of cultured zooxanthellae (CZ). Results showed that phosphorus-containing compounds observed in CZ were mainly phosphate and phosphate esters. Phosphate accounted for 19 ± 2% of the total phosphorus compounds observed in CZ maintained under low P-levels (0.02 μM). Adding 5 mM of dissolved inorganic phosphorus (KH2PO4) to the CZ culture medium led to a 3.1-fold increase in intracellular phosphate, while adding 5 mM of dissolved organic phosphorus led to a reduction in the concentration of phosphorus compounds, including a 2.5-fold intracellular phosphate decrease. In sharp contrast to zooxanthellae, the host mainly contained phosphonates, and to a lesser extent, phosphate esters and phosphate. Two-months of host starvation decreased the phosphate content by 2.4 fold, while bleaching of fed corals did not modify this content. Based on 31P NMR analyses, this study highlights the importance of phosphonates in the composition of coral host tissues, and illustrates the impact of phosphorus availability on the phosphorus composition of host tissues and CZ, both through feeding of the host and inorganic phosphorus enrichment of the CZ.