Screening of Genes Specifically Expressed in Males of Fenneropenaeus chinensis and Their Potential as Sex Markers
Screening of Genes Specifically Expressed in Males of Fenneropenaeus chinensis and Their Potential as Sex Markers
复制标题
中国明对虾雄性特异表达基因的筛选及其作为性别标记的潜力
DOI：
10.1155/2013/921067
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发表时间：
2013
影响因子：
--
通讯作者：
J. Xiang
中科院分区：
文献类型：
--
作者：
Shihao Li;Fuhua Li;Yusu Xie;B. Wang;R. Wen;Chengsong Zhang;Kuijie Yu;J. Xiang
The androgenic gland (AG), playing an important role in sex differentiation of male crustacean, is a target candidate to understand the mechanism of male development and to mine male-specific sex markers. An SSH library (designated as male reproduction-related tissues—SSH library, MRT-SSH library for short) was constructed using cDNA from tissues located at the basal part of the 5th pereiopods, including AG and part of spermatophore sac, as tester, and the cDNA from the basal part of the 4th pereiopods of these male shrimp as driver. 402 ESTs from the SSH library were sequenced and assembled into 48 contigs and 104 singlets. Twelve contigs and 14 singlets were identified as known genes. The proteins encoded by the identified genes were categorized, according to their proposed functions, into neuropeptide hormone and hormone transporter, RNA posttranscriptional regulation, translation, cell growth and death, metabolism, genetic information processing, signal transduction/transport, or immunity-related proteins. Eleven highly expressed contigs in the SSH library were selected for validation of the MRT-SSH library and screening sex markers of shrimp. One contig, specifically expressed in male shrimp, had a potential to be developed as a transcriptomic sex marker in shrimp.
软壳蛤核糖体蛋白 S19 的 cDNA 分离和 mRNA 表达表征。
影响因子：
3.5
作者：
Rhodes,LD;VanBeneden,RJ
通讯作者：
VanBeneden,RJ