Effects of light and temperature on the photoautotrophic growth and photoinhibition of nitrogen-fixing cyanobacterium Cyanothece sp ATCC 51142
Effects of light and temperature on the photoautotrophic growth and photoinhibition of nitrogen-fixing cyanobacterium Cyanothece sp ATCC 51142
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DOI：
10.1016/j.algal.2014.06.004
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发表时间：
2014-07-01
影响因子：
5.1
通讯作者：
Hellgardt, Klaus
中科院分区：
文献类型：
--
作者：
Dechatiwongse, Pongsathorn;Srisamai, Suna;Hellgardt, Klaus
单细胞、固氮蓝藻ATCC 51142是一种有前途的菌株，具有显著的产生大量氢气的能力，氢气是一种长期以来被推广为理想燃料的能量载体。在极端环境条件下，藻类和蓝藻的细胞光合能力通常会显著降低。甚至不太严格的条件也可以诱导光抑制生长动力学，这反过来又导致生物质和气体生产率的显著降低。在这项研究中，两个外部参数，即光强和温度，对蓝藻的光合自养生长的影响进行了详细的分析，以揭示关键条件，将导致不希望的光抑制。蓝藻生长和养分吸收动力学之间的高度一致性，以及强烈的依赖于这两个参数的变化。氮消耗被确认为触发器，其将指数生长期转化为稳定生长期。在320 μ E·m（-2）·s（-1）以下，最大比生长速率与辐照度之间存在非线性关系，并发现其主要受光饱和而非光抑制的影响。发现比生长速率与温度之间的关系是线性的，直到在40 ℃下观察到最终生物量生产率和蓝藻光合能力的显著下降。其原因是高温诱导的光抑制效应。(C)2014爱思唯尔有限公司版权所有。
The unicellular, nitrogen-fixing cyanobacterium Cyanothece sp. ATCC 51142 is a promising strain with a remarkable capability of producing large quantities of hydrogen, an energy carrier long being promoted as an ideal fuel. Under extreme environmental conditions, significant reduction of cellular photosynthetic capability is commonly observed in algae and cyanobacteria. Even less severe conditions can induce photo-inhibitive growth dynamics, which in turn result in a marked decrease in biomass and gas productivities. In this study a detailed analysis of the effect of two extrinsic parameters, namely light intensity and temperature, on the photoautotrophic growth of Cyanothece was performed in order to reveal critical conditions that would lead to undesired photoinhibition. A high degree of coherence between cyanobacterial growth and nutrient uptake kinetics was observed, as well as a strong dependence on the change of the two parameters. Nitrogen depletion was confirmed as a trigger, which transforms an exponential into a stationary growth phase. A non-linear relationship between the maximum specific growth rate and the irradiance up to 320 mu E m(-2) s(-1) was identified and found to be dominated by light saturation rather than photoinhibition. The relationship between the specific growth rate and the temperature was found to be linear until a remarkable drop in the final biomass productivity and cyanobacterial photosynthetic capability was observed at 40 degrees C. The cause of this is a high temperature-induced photoinhibition effect. (C) 2014 Elsevier B.V. All rights reserved.