Expression of amphiregulin in mucoepidermoid carcinoma of the major salivary glands: a molecular and clinicopathological study
Expression of amphiregulin in mucoepidermoid carcinoma of the major salivary glands: a molecular and clinicopathological study
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DOI：
10.1016/j.humpath.2016.06.016
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发表时间：
2016-11-01
期刊：
影响因子：
3.3
通讯作者：
Nibu, Ken-Ichi
中科院分区：
文献类型：
--
作者：
Shinomiya, Hitomi;Ito, Yohei;Nibu, Ken-Ichi
在粘液表皮样癌（MEC）中，CRTC 1-MAML 2融合表明预后良好。双调蛋白（AREG）是一种表皮生长因子受体（EGFR）配体，已被证明是CRTC 1-MAML 2融合的下游靶标，并在CRTC 1-MAML 2阳性MEC细胞系中的肿瘤生长和存活中发挥作用。本研究的目的是表征AREG和EGFR在大涎腺融合阳性和融合阴性MEC中的表达。应用免疫组化法检测33例大涎腺MEC中AREG和EGFR的表达。逆转录聚合酶链反应检测CRTC 1-MAML 2融合（23例CRTC 1-MAML 2融合阳性，10例融合阴性）。在23例融合阳性的病例中，17例（73.9%）和14例（60.9%）分别检测到AREG和EGFR过表达。在10例融合阴性的病例中，分别有1例（10%）和3例（30.0%）AREG和EGFR过表达。CRTC 1-MAML 2融合与AREG过表达呈正相关（P
In mucoepidermoid carcinoma (MEC), CRTC1-MAML2 fusion indicates a favorable prognosis. Amphiregulin (AREG), an epidermal growth factor receptor (EGFR) ligand, has been shown to be a downstream target of CRTC1-MAML2 fusion, and to play a role in tumor growth and survival in CRTC1-MAIVIL2 positive MEC cell lines. The aim of this study was to characterize the AREG and EGFR expression in the fusion-positive and fusion-negative MEC of the major salivary gland. The AREG and EGFR expression were studied by imrnunochemistty in 33 MEC cases of the major salivary glands. CRTC1-MAML2 fusion was tested by reverse-transcription polymerase chain reaction (23 CRTC1-MAML2 fusion-positive, 10 fusion negative). Of 23 fusion-positive cases, AREG and EGFR overexpression were detected in 17 (73.9%) and 14 (60.9%) cases, respectively. Of 10 fusion-negative cases, AREG and EGFR overexpression were detected in 1 (10%) and 3 (30.0%) cases, respectively. There was a positive correlation between CRTC1-MAML2 fusion and AREG overexpression (P