Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells
Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells
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DOI：
10.1111/j.1365-2591.2005.00987.x
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发表时间：
2005-09-01
影响因子：
5
通讯作者：
Coutinho, T
中科院分区：
文献类型：
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作者：
De Deus, G;Ximenes, R;Coutinho, T
目的评价两种矿物质三氧化物集合体(Pro-Root MTA(R)和MTA Angelus(R))和硅酸盐水泥(PC)对人血管内皮细胞株ECV 304的细胞毒作用。方法内皮ECV 304细胞在37℃、空气质量分数为95%、二氧化碳质量分数为5%、湿度为100%的环境中培养7d，在添加10%胎牛血清的F12培养液中培养。用比色法测定材料对线粒体功能的影响。在每个实验时间间隔(24、48和72h)，用四甲基四氮唑-二苯基四氮唑溴化法测定细胞活力。所有检测都重复三次，以确保重复性。结果用平均吸光度(A(570反斜杠，nm))+/-SD表示，数据用单因素方差分析和Bonferroni后验分析进行统计分析。结果两种实验材料之间无统计学差异(P>；0.05)。结论所分析的两种品牌的MTA和PC最初表现出相似的升高的细胞毒作用，但随着时间的推移逐渐减弱，使细胞培养得以重建。
Aim To evaluate the cytotoxic effects of two brands of mineral trioxide aggregate (MTA) (Pro-Root MTA((R)) and MTA Angelus((R))) and Portland cement (PC) on the human ECV 304 endothelial cell line.Methodology Endothelial ECV 304 cells were incubated at 37 degrees C in an atmosphere of 95% air, 5% carbon dioxide and 100% humidity for 7 days and grown in F12 medium supplemented with 10% fetal bovine serum with 50 mu g mL(-1) of gentamicin sulphate. Effects of the materials on mitochondrial functions were measured by a colorimetric assay. At each experimental time interval (24, 48 and 72 h), a dimethyl-thiazol-diphenyl tetrazolium bromid assay was conducted to measure cell viability. All assays were repeated three times to ensure reproducibility. Results were expressed as average absorbance (A(570 backslash,nm)) +/- SD and the data were analysed statistically by one-way analysis of variance and the Bonferroni post-test. A P-value < 0.05 was considered statistically significant.Results No statistically significant difference was shown between any of the experimental materials (P > 0.05).Conclusions The two brands of MTA analysed, as well as the PC, initially showed a similar elevated cytotoxic effect that decreased gradually with time allowing the cell culture to become reestablished.