Plasmonic SERS Au Nanosunflowers for Sensitive and Label-Free Diagnosis of DNA Base Damage in Stimulus-Induced Cell Apoptosis
Plasmonic SERS Au Nanosunflowers for Sensitive and Label-Free Diagnosis of DNA Base Damage in Stimulus-Induced Cell Apoptosis
复制标题
等离激元 SERS Au 纳米向日葵用于刺激诱导细胞凋亡中 DNA 碱基损伤的灵敏且无标记诊断
DOI：
10.1021/acs.analchem.0c01799
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发表时间：
2020-09-01
影响因子：
7.4
通讯作者：
Jin, Yongdong
中科院分区：
文献类型：
--
作者：
Qi, Guohua;Wang, Dandan;Jin, Yongdong
肿瘤细胞中复杂基因组DNA的分子诊断和准确的损伤分析对于癌症的治疗诊断至关重要，但仍然是一个巨大的挑战。本文中，通过设计制备均匀的等离子体向日葵状组装金（Au）纳米结构，该纳米结构能够有效地捕获DNA并提供高密度的间隙-等离子体“热点”用于足够的表面增强拉曼光谱（Sers）增强，我们首次成功地在DNA碱基水平上灵敏且可靠地检测电刺激诱导的凋亡癌细胞中的DNA损伤。Sers结果表明，外加电刺激（1.2V，5 min）对正常健康细胞几乎没有伤害，但会引起癌细胞DNA明显的双链断裂和腺嘌呤碱基损伤，从而有效地破坏DNA的复制和转录，最终诱导细胞凋亡。所开发的传感平台和方法对于遗传相关疾病的细胞研究是有希望的。
Molecular diagnosis and accurate damage analysis of complex genomic DNAs in tumor cells are crucial to the theranostics of cancers but still a huge challenge. Herein, by designed preparation of a uniform plasmonic sunflower-like assembly gold (Au) nanostructure that is capable of efficient DNA capture and providing high-density gap-plasmon "hot spots" for adequate surface-enhanced Raman spectroscopy (SERS) enhancement, we succeeded in sensitive and reliable label-free SERS detection of DNA damage in electrostimulus-induced apoptotic cancer cells at the DNA base level for the first time. The SERS results showed that the external electrostimulus (at 1.2 V, for 5 min) was almost harmless to normal healthy cells, but it caused pronounced double strand break and adenine base damage in cancer cell DNAs, which effectively destroyed the reproduction and transcription of DNAs and ultimately induced cell apoptosis. The developed sensing platform and method are promising for cell study of genetically related diseases.