Identification of OsMYC2-regulated senescence-associated genes in rice
Identification of OsMYC2-regulated senescence-associated genes in rice
复制标题
DOI：
10.1007/s00425-017-2697-5
复制
发表时间：
2017-04
期刊：
影响因子：
4.3
通讯作者：
Yuya Uji;K. Akimitsu;K. Gomi
中科院分区：
文献类型：
--
作者：
Yuya Uji;K. Akimitsu;K. Gomi
结论茉莉酸（jasmonic acid， JA）响应转录因子OsMYC2通过直接调控水稻叶片衰老相关基因，对叶片衰老起到正向调节作用。OsMYC2是一种转录因子（TF），是水稻茉莉酸（jasmonic acid， JA）信号通路的正调控因子，参与水稻的发育和防御。在此，我们报道了OsMYC2在黑暗诱导衰老（dark-induced senescence， DIS）条件下的叶片衰老中发挥重要作用。过表达osmyc2显著促进了水稻叶片衰老，表现为在DIS条件下叶绿素含量的降低。参与JA信号通路的水稻茉莉酸zim结构域蛋白OsJAZ8负调控了DIS条件下的叶片衰老。在体内，OsMYC2上调了一些衰老相关基因（sag）的表达，并选择性地结合了一些衰老相关基因启动子中的G-box/G-box样基序。这些结果表明，OsMYC2通过直接或间接调控水稻叶片衰老，起到了积极调节叶片衰老的作用。
Main conclusionThe jasmonic acid (JA)-responsive transcription factor OsMYC2 acts as a positive regulator of leaf senescence by direct regulation of some senescence-associated genes in rice.OsMYC2, a transcription factor (TF), acts as a positive regulator of jasmonic acid (JA) signaling involved in development and defense in rice. Here, we report that OsMYC2 plays an important role in leaf senescence under dark-induced senescence (DIS) conditions. Overexpression ofOsMYC2significantly promoted leaf senescence, indicated by reduction of chlorophyll content under DIS conditions in rice. Leaf senescence under the DIS conditions was negatively regulated by OsJAZ8, a rice jasmonate ZIM-domain protein involved in the JA signaling pathway. OsMYC2 upregulated the expression of some senescence-associated genes (SAGs) and selectively bound to the G-box/G-box-like motifs in the promoters of someSAGs in vivo. These results suggest that OsMYC2 acts as a positive regulator of leaf senescence by direct- or indirect-regulation ofSAGs in rice.