Microgravity Alters the Physiological Characteristics of Escherichia coli O157:H7 ATCC 35150, ATCC 43889, and ATCC 43895 under Different Nutrient Conditions

Microgravity Alters the Physiological Characteristics of Escherichia coli O157:H7 ATCC 35150, ATCC 43889, and ATCC 43895 under Different Nutrient Conditions
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DOI:
10.1128/aem.04037-13
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发表时间:
2014-04-01
影响因子:
4.4
通讯作者:
Rhee, M. S.
Rhee, M. S.
中科院分区:
生物学2区
文献类型:
--
作者:
Kim, H. W.;Matin, A.;Rhee, M. S.

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本研究的目的是了解微重力对在营养丰富或基本培养基中培养的重要食源性细菌大肠杆菌O 157:H7 ATCC 35150、ATCC 43889和ATCC 43895的影响。在低剪切模拟微重力(LSMMG;空间条件)和正常重力(NG;地球条件)下监测生理特性,如生长(通过光密度和平板培养测量)、细胞形态和pH值。在营养丰富的培养基中,除ATCC 35150外,所有菌株在LSMMG条件下培养6 h后的光密度均显著高于NG条件(P
The aim of this study is to provide understanding of microgravity effects on important food-borne bacteria, Escherichia coli O157:H7 ATCC 35150, ATCC 43889, and ATCC 43895, cultured in nutrient-rich or minimal medium. Physiological characteristics, such as growth (measured by optical density and plating), cell morphology, and pH, were monitored under low-shear modeled microgravity (LSMMG; space conditions) and normal gravity (NG; Earth conditions). In nutrient-rich medium, all strains except ATCC 35150 showed significantly higher optical density after 6 h of culture under LSMMG conditions than under NG conditions (P