Molecular and cellular specificity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family
Molecular and cellular specificity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family
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DOI：
10.1111/j.1742-4658.2009.07180.x
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发表时间：
2009-09-01
期刊：
影响因子：
5.4
通讯作者：
Soyez, Daniel
中科院分区：
文献类型：
--
作者：
Ollivaux, Celine;Gallois, Dominique;Soyez, Daniel
在多种动物，特别是脊椎动物和节肢动物的各种动物多肽中都检测到了D-氨基酰基残留物。这种不寻常的多态出现在美国龙虾甲壳类高血糖激素(CHH)的异构体中，因为在序列的第3位(CHH和d-Phe3 CHH)发现了d-苯丙氨基残基。在这项研究中，我们报告了在龙虾神经内分泌系统中，用于表征CHH家族另一成员的异构体的详细策略，卵黄发生抑制激素(VIH)。我们已经证明了第四个残基要么是L残基，要么是d-色氨酸残基(VIH和d-Trp4VIH)。此外，使用识别CHH和VIH同分异构体的抗血清表明，氨基酰异构化发生在X器官-鼻窦腺神经分泌系统的特殊细胞中，这两种神经肽的d型不仅存在于相同的细胞中，而且重要的是，它们共包装在相同的分泌小泡中。
d-aminoacyl residues have been detected in various animal peptides from several taxa, especially vertebrates and arthropods. This unusual polymorphism was shown to occur in isoforms of the crustacean hyperglycaemic hormone (CHH) of the American lobster because a d-phenylalanyl residue was found in position 3 of the sequence (CHH and d-Phe3 CHH). In the present study, we report the detailed strategy used to characterize, in the lobster neuroendocrine system, isomers of another member of the CHH family, vitellogenesis inhibiting hormone (VIH). We have demonstrated that the fourth residue is either an l- or a d- tryptophanyl residue (VIH and d-Trp4 VIH). Furthermore, use of antisera specifically recognizing the epimers of CHH and VIH reveals that aminoacyl isomerization occurs in specialized cells of the X organ-sinus gland neurosecretory system and that the d-forms of the two neuropeptides are not only present in the same cells, but, importantly, also are co-packaged within the same secretory vesicles.