In silico identification and expression analysis of Rare Cold Inducible 2 (RCI2) gene family in cucumber
In silico identification and expression analysis of Rare Cold Inducible 2 (RCI2) gene family in cucumber
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DOI：
10.1007/s13562-019-00510-6
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发表时间：
2019-06
影响因子：
1.9
通讯作者：
Yong Zhou;Lingli Ge;Guanghua Li;P. He;Yingui Yang;Shiqiang Liu
中科院分区：
文献类型：
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作者：
Yong Zhou;Lingli Ge;Guanghua Li;P. He;Yingui Yang;Shiqiang Liu
稀有冷诱导蛋白2（RCI 2），又称质膜蛋白3，是一类在植物生长发育和环境胁迫反应中起重要作用的低分子量蛋白。本研究对黄瓜（Cucumis sativusL.）的RCI 2基因家族进行了全面的分析，鉴定了4个RCI 2基因（CsaRCI 2 A-D），它们分布在黄瓜基因组的四条染色体上。4种CsaRCI 2蛋白氨基酸同源性在46.55%~ 76.00%之间。系统发育分析表明，黄瓜、拟南芥、蒺藜苜蓿、玉米和水稻的RCI 2蛋白可分为4个不同的进化枝，几乎所有的RCI 2基因都含有两个外显子，每个外显子之间有一个大小不等的内含子。在CsaRCI 2基因的启动子区发现了许多与发育、胁迫和死亡相关的顺式元件，并且CsaRCI 2基因似乎具有组织特异性表达模式。此外，qRT-PCR分析表明，这4个CsaRCI 2基因均对低温、盐、干旱等非生物胁迫以及阿坝处理有响应。本研究结果为进一步阐明RCI 2基因家族在黄瓜中的生物学功能奠定了基础。
Rare Cold Inducible 2 (RCI2), also known as plasma membrane protein 3, is a group of low molecular weight proteins that play crucial roles in plant growth and development as well as environmental stress responses. In this work, we performed a comprehensive analysis of theRCI2gene family and identified 4RCI2genes (CsaRCI2A–D) from cucumber (Cucumis sativusL.), which were distributed in four chromosomes of the cucumber genome. The amino acid identities among the 4 CsaRCI2 proteins varied from 46.55 to 76.00%. Phylogenetic analysis showed that RCI2 proteins from cucumber,Arabidopsis,Medicago truncatula, maize and rice could be classified into four distinct clades, and nearly all theRCI2genes harbored two exons separated by an intron of variable size. Many development-, stress-, and hormone-relatedcis-elements were found in the promoter regions of theCsaRCI2genes, and theCsaRCI2genes seemed to have tissue-specific expression patterns. In addition, qRT-PCR analysis revealed that these fourCsaRCI2genes were all responsive to various abiotic stresses including cold, salt, and drought, as well as ABA treatment. Our findings lay a foundation for further elucidating the biological roles of theRCI2gene family in cucumber.