A Fluorescence method to visualize the nuclear boundary by the lipophilic dye DiI.
A Fluorescence method to visualize the nuclear boundary by the lipophilic dye DiI.
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通过亲脂性染料 DiI 观察核边界的荧光方法。
DOI：
10.1080/09168451.2020.1756737
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
H. Saitoh
中科院分区：
文献类型：
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作者：
K. Miyazaki;K. Yano;H. Saitoh
在这里，我们描述了一个程序，荧光对比核边界使用亲脂性碳菁染料二碘在培养的人类细胞。本文的方法简单，适用于检测核边界缺陷，这可能与核膜动力学、微核形成和癌症生物学研究有关。缩写DiI：1，1 ′-dioctadecyl-3，3，3 ′，3 ′-tetramethylindocarbocyanine perchlorate; DiO：3，3 ′-dioctadecyloxacarbocyanine perchlorate; NE：nuclear envelope; RanBP 2：Ran-binding protein 2/Nucleoporin 358
Here, we describe a procedure to fluorescently contrast the nuclear boundary using the lipophilic carbocyanine dye DiI in cultured human cells. Our procedure is simple and is applicable to detect nuclear boundary defects, which may be relevant to studies on nuclear envelope dynamics, micronuclei formation and cancer biology.AbbreviationsDiI: 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate; DiO: 3,3ʹ-dioctadecyloxacarbocyanine perchlorate; NE: nuclear envelope; RanBP2: Ran-binding protein 2/Nucleoporin 358