Interaction between CD44 and hyaluronate induces chemoresistance in non-small cell lung cancer cell
Interaction between CD44 and hyaluronate induces chemoresistance in non-small cell lung cancer cell
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DOI：
10.1016/j.canlet.2006.12.025
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发表时间：
2007-07-18
期刊：
影响因子：
9.7
通讯作者：
Takahashi, Kazuhisa
中科院分区：
文献类型：
--
作者：
Ohashi, Rina;Takahashi, Fumiyuki;Takahashi, Kazuhisa
CD44s是一种主要的透明质酸(HA)受体，已被报道在癌细胞的侵袭和转移中发挥重要作用。本研究的目的是确定HA和CD44s之间的相互作用是否影响非小细胞肺癌(NSCLC)的体外化疗敏感性。转导CD44S基因(H322/CD44s)的NSCLC细胞株H322对顺铂(CDDP)的耐受性高于对牛血清白蛋白(BSA)的耐受性。用透明质酸诱导H322/CD44s细胞表达多药耐药蛋白2(MRP2)。MRP2抑制剂MK571不仅能抑制MRP2的表达，还能逆转CDDP耐药。这些结果提示CD44s和HA之间的相互作用在非小细胞肺癌获得性耐药中起着关键作用，MRP2可能参与了这一潜在机制。(C)2007爱思唯尔爱尔兰有限公司。保留所有权利。
CD44s is a principle hyaluronate (HA) receptor and has been reported to play an important role in cancer cell invasion and metastasis. The aim of our study is to determine if the interaction between HA and CD44s influences in vitro chemosensitivity of non-small cell lung cancer (NSCLC). NSCLC cell line, H322 cells, transfected with the CD44s gene (H322/ CD44s) cultured on HA coated plates were more resistant to cisplatin (CDDP) than that on bovine serum albumin. Multidrug resistance protein2 (MRP2) expression was induced in H322/CD44s cells cultured on HA. MRP2 inhibitor, MK571, not only suppressed MRP2 expression but also reversed CDDP resistance. These results suggest that the interaction between CD44s and HA play a pivotal role in acquired resistance to CDDP in NSCLC and MRP2 could be involved in this potential mechanism. (c) 2007 Elsevier Ireland Ltd. All rights reserved.